Cell Biology Antibodies 10

Anti-TPP2 Antibody (CAB6421)

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SKU:
CAB6421
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TPP2 Antibody
Antibody SKU:CAB6421
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TPP2 (NP_003282.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, SW620, HeLa, HepG2, Mouse brain, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TPP2 (NP_003282.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATA ATEE PFPF HGLL PKKE TGAA SFLC RYPE YDGR GVLI AVLD TGVD PGAP GMQV TTDG KPKI VDII DTTG SGDV NTAT EVEP KDGE IVGL SGRV LKIP ASWT NPSG KYHI GIKN GYDF YPKA LKER IQKE RKEK IWDP VHRV ALAE ACRK QEEF DVAN NGSS QANK LIKE ELQS QVEL LNSF EKKY SDPG PVYD CLVW HDGE VWRA CIDS NEDG DLSK STVL RNYK EAQE YGSF GTAE MLNY SVNI YDDG NLLS IVTS GGAH GTHV ASIA AGHF PEEP ERNG VAPG AQIL SIKI GDTR
Gene ID:7174
Uniprot:P29144
Cellular Location:Cytoplasm, Nucleus
Calculated MW:138kDa
Observed MW:138kDa
Synonyms:TPP2, TPP-2, TPP-II, TPPII
Background:This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class.
UniProt Protein Function:TPP2: Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Belongs to the peptidase S8 family.
UniProt Protein Details:

Protein type:EC 3.4.14.10; Protease

Chromosomal Location of Human Ortholog: 13q32-q33

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function:tripeptidyl-peptidase activity; serine-type endopeptidase activity; endopeptidase activity; aminopeptidase activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; protein polyubiquitination; proteolysis

NCBI Summary:This gene encodes a mammalian peptidase that, at neutral pH, removes tripeptides from the N terminus of longer peptides. The protein has a specialized function that is essential for some MHC class I antigen presentation. The protein is a high molecular mass serine exopeptidase; the amino acid sequence surrounding the serine residue at the active site is similar to the peptidases of the subtilisin class rather than the trypsin class. [provided by RefSeq, Jul 2008]
UniProt Code:P29144
NCBI GenInfo Identifier:34223721
NCBI Gene ID:7174
NCBI Accession:P29144.4
UniProt Secondary Accession:P29144,Q5VZU8,
UniProt Related Accession:P29144
Molecular Weight:1249
NCBI Full Name:Tripeptidyl-peptidase 2
NCBI Synonym Full Names:tripeptidyl peptidase II
NCBI Official Symbol:TPP2  
NCBI Official Synonym Symbols:TPP-2  
NCBI Protein Information:tripeptidyl-peptidase 2; TPP-II; tripeptidyl-peptidase II; tripeptidyl aminopeptidase
UniProt Protein Name:Tripeptidyl-peptidase 2
UniProt Synonym Protein Names:Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II
Protein Family:Tripeptidyl-peptidase
UniProt Gene Name:TPP2  
UniProt Entry Name:TPP2_HUMAN
Anti-TPP2 Antibody (CAB6421)Western blot analysis of extracts of various cell lines, using TPP2 antibody (CAB6421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TPP2 Antibody (CAB6421)Immunofluorescence analysis of U2OS cells using TPP2 antibody (CAB6421). Blue: DAPI for nuclear staining.
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