Name: Anti-TPM4 Antibody (CAB4166)
Brand: Cell Biology Antibodies 9
SKU: CAB4166
Availability: OutOfStock

Antibody Name:	Anti-TPM4 Antibody
Antibody SKU:	CAB4166
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human TPM4 (NP_003281.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human TPM4 (NP_003281.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGL NSLE AVKR KIQA LQQQ ADEA EDRA QGLQ RELD GERE RREK AEGD VAAL NRRI QLVE EELD RAQE RLAT ALQK LEEA EKAA DESE RGMK VIEN RAMK DEEK MEIQ EMQL KEAK HIAE EADR KYEE VARK LVIL EGEL ERAE ERAE VSEL KCGD LEEE LKNV TNNL KSLE AASE KYSE KEDK YEEE IKLL SDKL KEAE TRAE FAER TVAK LEKT IDDL EEKL AQAK EENV GLHQ TLDQ TLNE LNCI
Gene ID:	7171
Uniprot:	P67936
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	28kDa/32kDa
Observed MW:	36kDa

Synonyms:

TPM4, HEL-S-108

Background:

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TPM4: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In nonmuscle cells is implicated in stabilizing cytoskeleton actin filaments and endocytosis.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: cytoskeleton; cytosol; focal adhesion; membrane; muscle thin filament tropomyosin; stress fiber Molecular Function:protein binding; structural constituent of muscle Biological Process: cell motility; muscle contraction; muscle filament sliding; osteoblast differentiation
NCBI Summary:	This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009]
UniProt Code:	P67936
NCBI GenInfo Identifier:	54039751
NCBI Gene ID:	7171
NCBI Accession:	P67936.3
UniProt Secondary Accession:	P67936,P07226, Q15659, Q5U0D9, Q9BU85, Q9H8Q3, Q9UCS1 Q9UCS2, Q9UCS3, Q9UCS4,
UniProt Related Accession:	P67936
Molecular Weight:	32,723 Da
NCBI Full Name:	Tropomyosin alpha-4 chain
NCBI Synonym Full Names:	tropomyosin 4
NCBI Official Symbol:	TPM4
NCBI Official Synonym Symbols:	HEL-S-108
NCBI Protein Information:	tropomyosin alpha-4 chain
UniProt Protein Name:	Tropomyosin alpha-4 chain
UniProt Synonym Protein Names:	TM30p1; Tropomyosin-4
Protein Family:	Tropomyosin
UniProt Gene Name:	TPM4
UniProt Entry Name:	TPM4_HUMAN

Western blot analysis of extracts of various cell lines, using TPM4 antibody (CAB4166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

TPM4 Antibody (PACO12866)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)