Antibody Name:	Anti-TPM2 Antibody
Antibody SKU:	CAB3096
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human TPM2

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, HeLa, MCF7, U-87MG, Mouse skeletal muscle, Mouse lung

Immunogen:	A synthetic peptide of human TPM2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7169
Uniprot:	P07951
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	28kDa/32kDa
Observed MW:	36kDa

Synonyms:

TPM2, AMCD1, DA1, DA2B, HEL-S-273, NEM4, TMSB

Background:

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TPM2: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Defects in TPM2 are the cause of distal arthrogryposis type 1A (DA1A). A form of distal arthrogryposis, a disease characterized by congenital joint flexures or contractures that mainly involve the distal parts of the limbs, and affect two or more different body areas in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Belongs to the tropomyosin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 9p13 Cellular Component: cytosol; muscle thin filament tropomyosin Molecular Function:actin binding; structural constituent of muscle Biological Process: muscle contraction; muscle filament sliding; regulation of ATPase activity Disease: Arthrogryposis, Distal, Type 1a; Arthrogryposis, Distal, Type 2b; Myopathy, Congenital, With Fiber-type Disproportion; Nemaline Myopathy 4
NCBI Summary:	This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
UniProt Code:	P07951
NCBI GenInfo Identifier:	136090
NCBI Gene ID:	7169
NCBI Accession:	P07951.1
UniProt Secondary Accession:	P07951,P06468, Q13894, Q53FM4, Q5TCU4, Q5TCU7, Q9UH67 A6NM85,
UniProt Related Accession:	P07951
Molecular Weight:	28,684 Da
NCBI Full Name:	Tropomyosin beta chain
NCBI Synonym Full Names:	tropomyosin 2 (beta)
NCBI Official Symbol:	TPM2
NCBI Official Synonym Symbols:	DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273
NCBI Protein Information:	tropomyosin beta chain
UniProt Protein Name:	Tropomyosin beta chain
UniProt Synonym Protein Names:	Beta-tropomyosin; Tropomyosin-2
Protein Family:	Tropomyosin
UniProt Gene Name:	TPM2
UniProt Entry Name:	TPM2_HUMAN

	Western blot analysis of extracts of various cell lines, using TPM2 antibody (CAB3096) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using TPM2 antibody (CAB3096) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 300ug extracts of Mouse skeletal muscle cells using 3ug TPM2 antibody (CAB3096). Western blot was performed from the immunoprecipitate using TPM2 antibody (CAB3096) at a dilition of 1:1000.