Cell Biology Antibodies 6

Anti-TPCN1 Antibody (CAB15847)

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SKU:
CAB15847
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TPCN1 Antibody
Antibody SKU:CAB15847
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 692-816 of human TPCN1 (NP_060371.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 692-816 of human TPCN1 (NP_060371.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FRMN YSRK NQDS EVDG GITL EKEI SKEE LVAV LELY REAR GASS DVTR LLET LSQM ERYQ QHSM VFLG RRSR TKSD LSLK MYQE EIQE WYEE HARE QEQQ RQLS SSAA PAAQ QPPG SRQR SQTV T
Gene ID:53373
Uniprot:Q9ULQ1
Cellular Location:Endosome membrane, Lysosome membrane, Multi-pass membrane protein
Calculated MW:29kDa/94kDa/101kDa
Observed MW:94kDa
Synonyms:TPCN1, TPC1
Background:Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).
UniProt Protein Function:TPCN1: one of the major voltage-gated Ca(2+) channel (VDCC) across the plasma membrane. Highest expression found in the heart and kidney, and lowest expression found in the spleen. Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, integral; Transporter, ion channel; Transporter; Membrane protein, multi-pass; Channel, calcium

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: endosome; endosome membrane; integral to membrane; lysosomal membrane; lysosome; plasma membrane

Molecular Function:identical protein binding; protein binding; voltage-gated calcium channel activity

Biological Process: positive regulation of autophagy; release of sequestered calcium ion into cytosol

NCBI Summary:Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9ULQ1
NCBI GenInfo Identifier:29725622
NCBI Gene ID:53373
NCBI Accession:NP_060371.2
UniProt Secondary Accession:Q9ULQ1,Q86XS9, Q8NC20, A7E258,
UniProt Related Accession:Q9ULQ1
Molecular Weight:101,796 Da
NCBI Full Name:two pore calcium channel protein 1 isoform 2
NCBI Synonym Full Names:two pore segment channel 1
NCBI Official Symbol:TPCN1  
NCBI Official Synonym Symbols:TPC1  
NCBI Protein Information:two pore calcium channel protein 1
UniProt Protein Name:Two pore calcium channel protein 1
UniProt Synonym Protein Names:Voltage-dependent calcium channel protein TPC1
UniProt Gene Name:TPCN1  
UniProt Entry Name:TPC1_HUMAN
Anti-TPCN1 Antibody (CAB15847)Western blot analysis of extracts of rat lung, using TPCN1 antibody (CAB15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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