Epigenetics & Nuclear Signaling Antibodies 2
Anti-TP53RK Antibody (CAB14952)
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- SKU:
- CAB14952
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TP53RK Antibody |
| Antibody SKU: | CAB14952 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human TP53RK (NP_291028.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, BxPC-3, 293T, HeLa, Jurkat, Mouse thymus, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human TP53RK (NP_291028.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA RATT PADG EEPA PEAE ALAA ARER SSRF LSGL ELVK QGAE ARVF RGRF QGRA AVIK HRFP KGYR HPAL EARL GRRR TVQE ARAL LRCR RAGI SAPV VFFV DYAS NCLY MEEI EGSV TVRD YIQS TMET EKTP QGLS NLAK TIGQ VLAR MHDE DLIH GDLT TSNM LLKP PLEQ LNIV LIDF GLSF ISAL PEDK GVDL YVLE KAFL STHP NTET VFEA FLKS YSTS SKKA RPVL KKLD EVRL RGRK RSMV G |
| Gene ID: | 112858 |
| Uniprot: | Q96S44 |
| Cellular Location: | Nucleus |
| Calculated MW: | 28kDa |
| Observed MW: | 28kDa |
| Synonyms: | TP53RK, BUD32, C20orf64, Nori-2, Nori-2p, PRPK, dJ101A2 |
| Background: |
| UniProt Protein Function: | PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; Bud32 family Chromosomal Location of Human Ortholog: 20q13.2 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:p53 binding; protein binding; protein serine/threonine kinase activity Biological Process: protein amino acid phosphorylation |
| UniProt Code: | Q96S44 |
| NCBI GenInfo Identifier: | 26398348 |
| NCBI Gene ID: | 112858 |
| NCBI Accession: | Q96S44.2 |
| UniProt Secondary Accession: | Q96S44,Q3T977, Q5JZ01, Q6NZ60, Q96FM7, Q9NQE6, B3KU44 |
| UniProt Related Accession: | Q96S44 |
| Molecular Weight: | 28,160 Da |
| NCBI Full Name: | TP53-regulating kinase |
| NCBI Synonym Full Names: | TP53 regulating kinase |
| NCBI Official Symbol: | TP53RK |
| NCBI Official Synonym Symbols: | PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64 |
| NCBI Protein Information: | TP53-regulating kinase |
| UniProt Protein Name: | TP53-regulating kinase |
| UniProt Synonym Protein Names: | Atypical serine/threonine protein kinase TP53RK; EKC/KEOPS complex subunit TP53RK (EC:3.6.-.-); Nori-2; p53-related protein kinase |
| Protein Family: | TP53-regulating kinase |
| UniProt Gene Name: | TP53RK |
| UniProt Entry Name: | PRPK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TP53RK antibody (CAB14952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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