Cell Biology Antibodies 11

Anti-TP53I3 Antibody (CAB7576)

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SKU:
CAB7576
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TP53I3 Antibody
Antibody SKU:CAB7576
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 163-332 of human TP53I3 (NP_671713.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, SW480, SW620, HepG2, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 163-332 of human TP53I3 (NP_671713.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGA IPLV TAGS QKKL QMAE KLGA AAGF NYKK EDFS EATL KFTK GAGV NLIL DCIG GSYW EKNV NCLA LDGR WVLY GLMG GGDI NGPL FSKL LFKR GSLI TSLL RSRD NKYK QMLV NAFT EQIL PHFS TEGP QRLL PVLD RIYP VTEI QEAH KYME ANKN IGKI VLEL PQ
Gene ID:9540
Uniprot:Q53FA7
Cellular Location:
Calculated MW:25kDa/35kDa
Observed MW:37kDa/72kDa
Synonyms:TP53I3, PIG3
Background:The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TP53I3: May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro). Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p23.3

Molecular Function:NADPH:quinone reductase activity; protein homodimerization activity; quinone binding; zinc ion binding

Biological Process: NADP metabolic process

NCBI Summary:The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
UniProt Code:Q53FA7
NCBI GenInfo Identifier:76789665
NCBI Gene ID:9540
NCBI Accession:Q53FA7.2
UniProt Secondary Accession:Q53FA7,O14679, O14685, Q38G78, Q6JLE7, Q9BWB8, D6W533
UniProt Related Accession:Q53FA7
Molecular Weight:25,402 Da
NCBI Full Name:Quinone oxidoreductase PIG3
NCBI Synonym Full Names:tumor protein p53 inducible protein 3
NCBI Official Symbol:TP53I3  
NCBI Official Synonym Symbols:PIG3  
NCBI Protein Information:quinone oxidoreductase PIG3
UniProt Protein Name:Quinone oxidoreductase PIG3
UniProt Synonym Protein Names:Tumor protein p53-inducible protein 3; p53-induced gene 3 protein
Protein Family:Quinone oxidoreductase
UniProt Gene Name:TP53I3  
UniProt Entry Name:QORX_HUMAN
Anti-TP53I3 Antibody (CAB7576)Western blot analysis of extracts of various cell lines, using TP53I3 antibody (CAB7576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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