Anti-TORC2 Antibody (CAB20893)

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SKU:
CAB20893
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-TORC2 Antibody (CAB20893)
Antibody SKU:CAB20893
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of Human TORC2.
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:293T, Rat liver
Immunogen:Recombinant protein of Human TORC2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Cytoplasm, Nucleus
Calculated MW:73kDa
Observed MW:80KDa
Synonyms:CRTC2, TORC-2, TORC2
Background:This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5
Western blot analysis of extracts of Rat liver, using TORC2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.Western blot analysis of extracts of Rat liver, using TORC2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Western blot analysis of extracts of 293T cells, using TORC2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Western blot analysis of extracts of 293T cells, using TORC2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
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