Epigenetics & Nuclear Signaling Antibodies 1

Anti-TOPBP1 Antibody (CAB12566)

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SKU:
CAB12566
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TOPBP1 Antibody
Antibody SKU:CAB12566
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse ovary, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1253-1522 of human TOPBP1 (NP_008958.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KIIT IEET HEEL KKQY IFQL SSLN PQER IDYC HLIE KLGG LVIE KQCF DPTC THIV VGHP LRNE KYLA SVAA GKWV LHRS YLEA CRTA GHFV QEED YEWG SSSI LDVL TGIN VQQR RLAL AAMR WRKK IQQR QESG IVEG AFSG WKVI LHVD QSRE AGFK RLLQ SGGA KVLP GHSV PLFK EATH LFSD LNKL KPDD SGVN IAEA AAQN VYCL RTEY IADY LMQE SPPH VENY CLPE AISF IQNN KELG TGLS QKRK APTE KNKI KRPR VH
Gene ID:11073
Uniprot:Q92547
Cellular Location:Chromosome, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:170kDa
Observed MW:130kDa
Synonyms:TOPBP1, TOP2BP1
Background:This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
UniProt Protein Function:TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.
UniProt Protein Details:

Protein type:DNA replication

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: male germ cell nucleus; Golgi apparatus; spindle pole; centrosome; PML body; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton

Molecular Function:protein C-terminus binding; identical protein binding; protein binding; DNA binding

Biological Process: response to ionizing radiation; DNA repair; response to DNA damage stimulus; DNA metabolic process

NCBI Summary:This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
UniProt Code:Q92547
NCBI GenInfo Identifier:296453012
NCBI Gene ID:11073
NCBI Accession:Q92547.3
UniProt Secondary Accession:Q92547,Q7LGC1, Q9UEB9, B7Z7W8,
UniProt Related Accession:Q92547
Molecular Weight:1522
NCBI Full Name:DNA topoisomerase 2-binding protein 1
NCBI Synonym Full Names:topoisomerase (DNA) II binding protein 1
NCBI Official Symbol:TOPBP1  
NCBI Official Synonym Symbols:TOP2BP1  
NCBI Protein Information:DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-binding protein 1; DNA topoisomerase II-beta-binding protein 1
UniProt Protein Name:DNA topoisomerase 2-binding protein 1
UniProt Synonym Protein Names:DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1
Protein Family:DNA topoisomerase 2-binding protein
UniProt Gene Name:TOPBP1  
UniProt Entry Name:TOPB1_HUMAN
Anti-TOPBP1 Antibody (CAB12566)Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (CAB12566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 50s.
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