Name: Anti-TOP3B Antibody (CAB8469)
Brand: Cell Biology Antibodies 12
SKU: CAB8469
Availability: OutOfStock

Antibody Name:	Anti-TOP3B Antibody
Antibody SKU:	CAB8469
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TOP3B (NP_003926.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	293T, A-431, HT-1080, BT-474, HL-60, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TOP3B (NP_003926.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKTV LMVA EKPS LAQS IAKI LSRG SLSS HKGL NGAC SVHE YTGT FAGQ PVRF KMTS VCGH VMTL DFLG KYNK WDKV DPAE LFSQ APTE KKEA NPKL NMVK FLQV EGRG CDYI VLWL DCDK EGEN ICFE VLDA VLPV MNKA HGGE KTVF RARF SSIT DTDI CNAM ACLG EPDH NEAL SVDA RQEL DLRI GCAF TRFQ TKYF QGKY GDLD SSLI SFGP CQTP TLGF CVER HDKI QSFK PETY WVLQ AKVN TDKD RSLL LDWD RVRV FDRE IA
Gene ID:	8940
Uniprot:	O95985
Cellular Location:
Calculated MW:	79kDa/82kDa/96kDa
Observed MW:	120kDa

Synonyms:

TOP3B, TOP3B1

Background:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

UniProt Protein Function:	TOP3B: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity. Belongs to the type IA topoisomerase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; EC 5.99.1.2; Topoisomerase Chromosomal Location of Human Ortholog: 22q11.22 Cellular Component: nucleus Molecular Function:DNA topoisomerase activity; protein binding
NCBI Summary:	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
UniProt Code:	O95985
NCBI GenInfo Identifier:	6686033
NCBI Gene ID:	8940
NCBI Accession:	O95985.1
UniProt Secondary Accession:	O95985,Q9BUP5, A0M8Q3,
UniProt Related Accession:	O95985
Molecular Weight:	79,839 Da
NCBI Full Name:	DNA topoisomerase 3-beta-1
NCBI Synonym Full Names:	topoisomerase (DNA) III beta
NCBI Official Symbol:	TOP3B
NCBI Official Synonym Symbols:	TOP3B1
NCBI Protein Information:	DNA topoisomerase 3-beta-1
UniProt Protein Name:	DNA topoisomerase 3-beta-1
UniProt Synonym Protein Names:	DNA topoisomerase III beta-1
Protein Family:	DNA topoisomerase
UniProt Gene Name:	TOP3B
UniProt Entry Name:	TOP3B_HUMAN

Anti-TOP3B Polyclonal Antibody (CAB8469)

Western blot analysis of extracts of various cell lines, using TOP3B antibody (CAB8469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

TOP3B Antibody (PACO01645)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)