Antibody Name:	Anti-TOP3A Antibody
Antibody SKU:	CAB16988
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP3A (XP_011522303.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP3A (XP_011522303.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MILA RNYL DVYP YDHW SDKI LPVY EQGS HFQP STVE MVDG ETSP PKLL TEAD LIAL MEKH GIGT DATH AEHI ETIK ARMY VGLT PDKR FLPG HLGM GLVE
Gene ID:	7156
Uniprot:	Q13472
Cellular Location:
Calculated MW:	101kDa/109kDa/112kDa
Observed MW:	Refer to figures

Synonyms:

TOP3A, TOP3, ZGRF7

Background:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.
NCBI Summary:	This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Code:	Q13472
NCBI GenInfo Identifier:	1002819406
NCBI Gene ID:	7156
NCBI Accession:	NP_001307688.1
UniProt Secondary Accession:	Q13472,Q13473, A8KA61, B4DK80, D3DXC7,
UniProt Related Accession:	Q13472
Molecular Weight:	101,208 Da
NCBI Full Name:	DNA topoisomerase 3-alpha isoform 2
NCBI Synonym Full Names:	DNA topoisomerase III alpha
NCBI Official Symbol:	TOP3A
NCBI Official Synonym Symbols:	TOP3; ZGRF7
NCBI Protein Information:	DNA topoisomerase 3-alpha
UniProt Protein Name:	DNA topoisomerase 3-alpha
UniProt Synonym Protein Names:	DNA topoisomerase III alpha
UniProt Gene Name:	TOP3A