Cell Biology Antibodies 6
Anti-TOP1MT Antibody (CAB15559)
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- SKU:
- CAB15559
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TOP1MT Antibody |
| Antibody SKU: | CAB15559 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RATP STFE KSMQ NLQT KIQA KKEQ VAEA RAEL RRAR AEHK AQGD GKSR SVLE KKRR LLEK LQEQ LAQL SVQA TDKE ENKQ V |
| Gene ID: | 116447 |
| Uniprot: | Q969P6 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 58kDa/69kDa |
| Observed MW: | 70kDa |
| Synonyms: | TOP1MT |
| Background: | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
| NCBI Summary: | This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | Q969P6 |
| NCBI GenInfo Identifier: | 386642871 |
| NCBI Gene ID: | 116447 |
| NCBI Accession: | NP_001245375.1 |
| UniProt Secondary Accession: | Q969P6,Q86ST4, Q86V82, B7ZAR5, E7ES89, |
| UniProt Related Accession: | Q969P6 |
| Molecular Weight: | 66 |
| NCBI Full Name: | DNA topoisomerase I, mitochondrial isoform 2 |
| NCBI Synonym Full Names: | DNA topoisomerase I mitochondrial |
| NCBI Official Symbol: | TOP1MT |
| NCBI Protein Information: | DNA topoisomerase I, mitochondrial |
| UniProt Protein Name: | DNA topoisomerase I, mitochondrial |
| UniProt Gene Name: | TOP1MT |
 | Western blot analysis of extracts of A-549 cells, using TOP1MT antibody (CAB15559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60S. |
 | Immunohistochemistry of paraffin-embedded Human colon using TOP1MT Rabbit pAb (CAB15559) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat lung using TOP1MT Rabbit pAb (CAB15559) at dilution of 1:100 (40x lens). |
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