Signal Transduction Antibodies 2
Anti-TOMM40 Antibody (CAB3213)
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- SKU:
- CAB3213
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-TOMM40 Antibody |
| Antibody SKU: | CAB3213 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-2OS, HepG2, Mouse brain, Mouse testis, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human TOMM40 (NP_001122389.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGNV LAAS SPPA GPPP PPAP ALVG LPPP PPSP PGFT LPPL GGSL GAGT STSR SSER TPGA ATAS ASGA AEDG ACGC LPNP GTFE ECHR KC |
| Gene ID: | 10452 |
| Uniprot: | O96008 |
| Cellular Location: | Mitochondrion outer membrane, Multi-pass membrane protein |
| Calculated MW: | 34kDa/37kDa |
| Observed MW: | 38kDa |
| Synonyms: | TOMM40, C19orf1, D19S1177E, PER-EC1, PEREC1, TOM40 |
| Background: | The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | TOMM40: Channel-forming protein essential for import of protein precursors into mitochondria. Belongs to the Tom40 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Mitochondrial Chromosomal Location of Human Ortholog: 19q13 Cellular Component: nucleoplasm; pore complex; mitochondrial outer membrane translocase complex; mitochondrion; cytoplasm; integral to membrane; integral to mitochondrial outer membrane Molecular Function:protein transmembrane transporter activity; porin activity Biological Process: cellular protein metabolic process; ion transport; protein targeting to mitochondrion |
| NCBI Summary: | The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015] |
| UniProt Code: | O96008 |
| NCBI GenInfo Identifier: | 12230369 |
| NCBI Gene ID: | 10452 |
| NCBI Accession: | O96008.1 |
| UniProt Related Accession: | O96008 |
| Molecular Weight: | Observed MW: 38kDa |
| NCBI Full Name: | Mitochondrial import receptor subunit TOM40 homolog |
| NCBI Synonym Full Names: | translocase of outer mitochondrial membrane 40 |
| NCBI Official Symbol: | TOMM40 |
| NCBI Official Synonym Symbols: | TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E |
| NCBI Protein Information: | mitochondrial import receptor subunit TOM40 homolog |
| UniProt Protein Name: | Mitochondrial import receptor subunit TOM40 homolog |
| UniProt Synonym Protein Names: | Protein Haymaker; Translocase of outer membrane 40 kDa subunit homolog; p38.5 |
| Protein Family: | Mitochondrial import receptor |
| UniProt Gene Name: | TOMM40 |
| UniProt Entry Name: | TOM40_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOMM40 antibody (CAB3213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Confocal immunofluorescence analysis of U2OS cells using TOMM40 Polyclonal Antibody (CAB3213) at dilution of 1:200. Blue: DAPI for nuclear staining. |
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