Cell Biology Antibodies 5
Anti-TOMM22 Antibody (CAB14548)
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- SKU:
- CAB14548
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TOMM22 Antibody |
| Antibody SKU: | CAB14548 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-83 of human TOMM22 (NP_064628.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, HepG2, K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-83 of human TOMM22 (NP_064628.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAA VAAA GAGE PQSP DELL PKGD AEKP EEEL EEDD DEEL DETL SERL WGLT EMFP ERVR SAAG ATFD LSLF VAQK MYRF SRA |
| Gene ID: | 56993 |
| Uniprot: | Q9NS69 |
| Cellular Location: | Mitochondrion outer membrane, Single-pass membrane protein |
| Calculated MW: | 15kDa |
| Observed MW: | 16kDa |
| Synonyms: | TOMM22, 1C9-2, MST065, MSTP065, TOM22 |
| Background: | The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. |
| UniProt Protein Function: | TOMM22: Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Belongs to the Tom22 family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Membrane protein, integral Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: mitochondrion; membrane; mitochondrial outer membrane translocase complex; mitochondrial inner membrane; integral to membrane Molecular Function:protein binding; protein transmembrane transporter activity Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane |
| NCBI Summary: | The protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NS69 |
| NCBI GenInfo Identifier: | 24212074 |
| NCBI Gene ID: | 56993 |
| NCBI Accession: | Q9NS69.3 |
| UniProt Secondary Accession: | Q9NS69,Q9CPQ3, |
| UniProt Related Accession: | Q9NS69 |
| Molecular Weight: | 142 |
| NCBI Full Name: | Mitochondrial import receptor subunit TOM22 homolog |
| NCBI Synonym Full Names: | translocase of outer mitochondrial membrane 22 homolog (yeast) |
| NCBI Official Symbol: | TOMM22 |
| NCBI Official Synonym Symbols: | 1C9-2; TOM22; MST065; MSTP065 |
| NCBI Protein Information: | mitochondrial import receptor subunit TOM22 homolog; mitochondrial import receptor Tom22; translocase of outer membrane 22 kDa subunit homolog |
| UniProt Protein Name: | Mitochondrial import receptor subunit TOM22 homolog |
| UniProt Synonym Protein Names: | 1C9-2; Translocase of outer membrane 22 kDa subunit homolog |
| Protein Family: | Mitochondrial import receptor |
| UniProt Gene Name: | TOMM22 |
| UniProt Entry Name: | TOM22_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOMM22 antibody (CAB14548) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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