KO Validated Antibodies 1

Anti-TOM20 Antibody (CAB18047)[KO Validated]

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SKU:
CAB18047
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TOM20 Antibody [KO Validated]
Antibody SKU:CAB18047
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human TOMM20.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, THP-1, 293T, Mouse liver, Mouse brain, Rat kidney
Immunogen:Recombinant protein of human TOMM20.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9804
Uniprot:Q15388
Cellular Location:Mitochondrion outer membrane, Single-pass membrane protein
Calculated MW:16kDa
Observed MW:16KDa
Anti-TOM20 Antibody [KO Validated] (CAB18047)Western blot analysis of extracts from normal (control) and TOMM20 knockout (KO) 293T cells, using TOMM20 antibody (CAB18047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-TOM20 Antibody [KO Validated] (CAB18047)Western blot analysis of extracts of various cell lines, using TOM20 antibody (CAB18047) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-TOM20 Antibody [KO Validated] (CAB18047)Western blot analysis of extracts of various cell lines, using TOM20 antibody (CAB18047) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-TOM20 Antibody [KO Validated] (CAB18047)Immunofluorescence analysis of C6 cells using [KO Validated] TOM20 Rabbit pAb (CAB18047) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-TOM20 Antibody [KO Validated] (CAB18047)Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] TOM20 Rabbit pAb (CAB18047) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synonyms:TOMM20, MAS20, MOM19, TOM20
Background:
UniProt Protein Function:TOM20: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Belongs to the Tom20 family.
UniProt Protein Details:

Protein type:Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: mitochondrial outer membrane translocase complex; mitochondrion; integral to membrane

Molecular Function:protein binding; P-P-bond-hydrolysis-driven protein transmembrane transporter activity; unfolded protein binding

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion

UniProt Code:Q15388
NCBI GenInfo Identifier:2498697
NCBI Gene ID:9804
NCBI Accession:Q15388.1
UniProt Secondary Accession:Q15388,Q498B3, Q6IBT4, A8K195,
UniProt Related Accession:Q15388,AAB35420
Molecular Weight:145
NCBI Full Name:Mitochondrial import receptor subunit TOM20 homolog
NCBI Synonym Full Names:translocase of outer mitochondrial membrane 20 homolog (yeast)
NCBI Official Symbol:TOMM20  
NCBI Official Synonym Symbols:MAS20; MOM19; TOM20  
NCBI Protein Information:mitochondrial import receptor subunit TOM20 homolog; mitochondrial 20 kDa outer membrane protein; outer mitochondrial membrane receptor Tom20; translocase of outer mitochondrial membrane 20 homolog type II
UniProt Protein Name:Mitochondrial import receptor subunit TOM20 homolog
UniProt Synonym Protein Names:Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20
Protein Family:TOMM20-like protein
UniProt Gene Name:TOMM20  
UniProt Entry Name:TOM20_HUMAN
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