Autophagy Antibodies

Anti-TOLLIP Antibody (CAB2202)

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SKU:
CAB2202
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TOLLIP Antibody
Antibody SKU:CAB2202
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, OVCAR3, MCF7, 293T, Mouse spleen, Mouse intestine, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human TOLLIP (NP_061882.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATT VSTQ RGPV YIGE LPQD FLRI TPTQ QQRQ VQLD AQAA QQLQ YGGA VGTV GRLN ITVV QAKL AKNY GMTR MDPY CRLR LGYA VYET PTAH NGAK NPRW NKVI HCTV PPGV DSFY LEIF DERA FSMD DRIA WTHI TIPE SLRQ GKVE DKWY SLSG RQGD DKEG MINL VMSY ALLP AAMV MPPQ PVVL MPTV YQQG VGYV PITG MPAV CSPG MVPV ALPP AAVN AQPR CSEE DLKA IQDM FPNM DQEV IRSV LEAQ RGNK DAAI NSLL QMGE EP
Gene ID:54472
Uniprot:Q9H0E2
Cellular Location:Cytoplasm
Calculated MW:22kDa/30kDa
Observed MW:30kDa
Synonyms:TOLLIP, IL-1RAcPIP
Background:This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.
UniProt Protein Details:

Protein type:Vesicle; Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; cytosol

Molecular Function:kinase binding; protein binding

Biological Process: cell-cell signaling; epithelial cell differentiation; phosphorylation; signal transduction

NCBI Summary:This gene encodes a ubiquitin-binding protein that interacts with several Toll-like receptor (TLR) signaling cascade components. The encoded protein regulates inflammatory signaling and is involved in interleukin-1 receptor trafficking and in the turnover of IL1R-associated kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
UniProt Code:Q9H0E2
NCBI GenInfo Identifier:20140803
NCBI Gene ID:54472
NCBI Accession:Q9H0E2.1
UniProt Secondary Accession:Q9H0E2,Q9H9E6, Q9UJ69, B3KXC6,
UniProt Related Accession:Q9H0E2
Molecular Weight:22,812 Da
NCBI Full Name:Toll-interacting protein
NCBI Synonym Full Names:toll interacting protein
NCBI Official Symbol:TOLLIP  
NCBI Official Synonym Symbols:IL-1RAcPIP  
NCBI Protein Information:toll-interacting protein
UniProt Protein Name:Toll-interacting protein
Protein Family:Toll-interacting protein
UniProt Gene Name:TOLLIP  
UniProt Entry Name:TOLIP_HUMAN
Anti-TOLLIP Antibody (CAB2202)Western blot analysis of extracts of various cell lines, using TOLLIP antibody (CAB2202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-TOLLIP Antibody (CAB2202)Immunohistochemistry of paraffin-embedded rat brain using TOLLIP antibody (CAB2202) at dilution of 1:100 (40x lens).
Anti-TOLLIP Antibody (CAB2202)Immunofluorescence analysis of L929 cells using TOLLIP antibody (CAB2202) at dilution of 1:100. Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman TOLLIP Recombinant Protein Toll-Interacting Protein (TOLLIP) is a member of the tollip family. TOLLIP localizes to the cytoplasm. It contains one C2 domain and one