Cell Biology Antibodies 11
Anti-TOB2 Antibody (CAB7223)
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- SKU:
- CAB7223
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TOB2 Antibody |
| Antibody SKU: | CAB7223 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TOB2 (NP_057356.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, 293T, MCF7, Rat skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TOB2 (NP_057356.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQLE IKVA LNFI ISYL YNKL PRRR ADLF GEEL ERLL KKKY EGHW YPEK PLKG SGFR CVHI GEMV DPVV EL |
| Gene ID: | 10766 |
| Uniprot: | Q14106 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 27kDa/36kDa |
| Observed MW: | 37kDa |
| Synonyms: | TOB2, APRO5, TOB4, TOBL, TROB2 |
| Background: |
| UniProt Protein Function: | TOB2: Anti-proliferative protein inhibits cell cycle progression from the G0/G1 to S phases. Belongs to the BTG family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: nucleus Molecular Function:protein binding Biological Process: female gamete generation |
| NCBI Summary: | TOB2 belongs to the TOB (see TOB1; MIM 605523)/BTG1 (MIM 109580) family of antiproliferative proteins, which are involved in the regulation of cell cycle progression.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q14106 |
| NCBI GenInfo Identifier: | 12643431 |
| NCBI Gene ID: | 10766 |
| NCBI Accession: | Q14106.2 |
| UniProt Secondary Accession: | Q14106,Q6FHR7, Q6PIT9, Q9BY97, Q9UBI0, |
| UniProt Related Accession: | Q14106 |
| Molecular Weight: | 27,192 Da |
| NCBI Full Name: | Protein Tob2 |
| NCBI Synonym Full Names: | transducer of ERBB2, 2 |
| NCBI Official Symbol: | TOB2 |
| NCBI Official Synonym Symbols: | TOB4; TOBL; APRO5; TROB2 |
| NCBI Protein Information: | protein Tob2 |
| UniProt Protein Name: | Protein Tob2 |
| UniProt Synonym Protein Names: | Protein Tob4; Transducer of erbB-2 2 |
| Protein Family: | Protein |
| UniProt Gene Name: | TOB2 |
| UniProt Entry Name: | TOB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TOB2 antibody (CAB7223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using TOB2 antibody (CAB7223) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using TOB2 antibody (CAB7223) at dilution of 1:100 (40x lens). |
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