Cell Biology Antibodies 1

Anti-TNNT1 Antibody (CAB10354)

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SKU:
CAB10354
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TNNT1 Antibody
Antibody SKU:CAB10354
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human TNNT1 (NP_001119604.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, A-549, SW480
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human TNNT1 (NP_001119604.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSDT EEQE YEEE QPEE EAAE EEEE APEE PEPV AEPE EERP KPSR PVVP PLIP PKIP EGER VDFD DIHR KRME KDLL ELQT LIDV HFEQ RKKE EEEL VALK ERIE RRRS ERAE QQRF RTEK ERER QAKL AEEK MRKE EEEA KKRA EDDA KKKK VLSN MGAH FGGY LVKA EQKR GKRQ TGRE MKVR ILSE RKKP LDID YMGE EQLR EKAQ ELSD WIHQ LESE KFDL MAKL KQQK YEIN VLYN RISH AQKF RKGA GKGR VGGR WK
Gene ID:7138
Uniprot:P13805
Cellular Location:
Calculated MW:30kDa/31kDa/32kDa
Observed MW:33kDa
Synonyms:TNNT1, ANM, NEM5, STNT, TNT, TNTS, troponin T1
Background:This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TNNT1: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT1 are the cause of nemaline myopathy type 5 (NEM5); also known as Amish nemaline myopathy (ANM). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 5 is a severe and progressive form common among Old Order Amish. Affected infants display tremors with hypotonia and mild contractures of the shoulders and hips. Proximal contractures progressively weaken and a pectus carinatum deformity develops before children die of respiratory insufficiency, usually in the second year. Belongs to the troponin T family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytosol; troponin complex

Molecular Function:protein binding; tropomyosin binding; troponin T binding

Biological Process: muscle filament sliding; negative regulation of muscle contraction; skeletal muscle contraction; slow-twitch skeletal muscle fiber contraction; transition between fast and slow fiber

Disease: Nemaline Myopathy 5

NCBI Summary:This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P13805
NCBI GenInfo Identifier:1174800
NCBI Gene ID:7138
NCBI Accession:P13805.4
UniProt Secondary Accession:P13805,O95472, Q16061, Q5U0E1,
UniProt Related Accession:P13805
Molecular Weight:31,242 Da
NCBI Full Name:Troponin T, slow skeletal muscle
NCBI Synonym Full Names:troponin T1, slow skeletal type
NCBI Official Symbol:TNNT1  
NCBI Official Synonym Symbols:ANM; TNT; NEM5; STNT; TNTS  
NCBI Protein Information:troponin T, slow skeletal muscle
UniProt Protein Name:Troponin T, slow skeletal muscle
UniProt Synonym Protein Names:Slow skeletal muscle troponin T; sTnT
Protein Family:Troponin
UniProt Gene Name:TNNT1  
UniProt Entry Name:TNNT1_HUMAN
Anti-TNNT1 Antibody (CAB10354)Western blot analysis of extracts of various cell lines, using TNNT1 antibody (CAB10354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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