Cell Biology Antibodies 11
Anti-TNNI3K Antibody (CAB7802)
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- SKU:
- CAB7802
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TNNI3K Antibody |
| Antibody SKU: | CAB7802 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human TNNI3K (NP_057062.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Mouse thymus, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human TNNI3K (NP_057062.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGNY KSRP TQTC TDEW KKKV SESY VITI ERLE DDLQ IKEK ELTE LRNI FGSD EAFS KVNL NYRT ENGL SLLH LCC |
| Gene ID: | 51086 |
| Uniprot: | Q59H18 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 77kDa/92kDa/94kDa/104kDa |
| Observed MW: | 93kDa |
| Synonyms: | TNNI3K, CARK, CCDD |
| Background: | This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology. |
| UniProt Protein Function: | May play a role in cardiac physiology. |
| NCBI Summary: | This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010] |
| UniProt Code: | Q59H18 |
| NCBI GenInfo Identifier: | 510785758 |
| NCBI Gene ID: | 100526835 |
| NCBI Accession: | NP_001186256.2 |
| UniProt Secondary Accession: | Q59H18,Q17RN0, Q49AR1, Q6MZS9, Q9Y2V6, |
| Molecular Weight: | 104 kDa |
| NCBI Full Name: | FPGT-TNNI3K fusion protein isoform b |
| NCBI Synonym Full Names: | FPGT-TNNI3K readthrough |
| NCBI Official Symbol: | FPGT-TNNI3K |
| NCBI Official Synonym Symbols: | CARK; TNNI3K |
| NCBI Protein Information: | FPGT-TNNI3K fusion protein |
| UniProt Protein Name: | Serine/threonine-protein kinase TNNI3K |
| UniProt Synonym Protein Names: | Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase |
| UniProt Gene Name: | TNNI3K |
 | Western blot analysis of extracts of various cell lines, using TNNI3K antibody (CAB7802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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