Cell Biology Antibodies 11

Anti-TNNI3K Antibody (CAB7802)

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SKU:
CAB7802
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TNNI3K Antibody
Antibody SKU:CAB7802
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human TNNI3K (NP_057062.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, HepG2, Mouse thymus, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human TNNI3K (NP_057062.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGNY KSRP TQTC TDEW KKKV SESY VITI ERLE DDLQ IKEK ELTE LRNI FGSD EAFS KVNL NYRT ENGL SLLH LCC
Gene ID:51086
Uniprot:Q59H18
Cellular Location:Cytoplasm, Nucleus
Calculated MW:77kDa/92kDa/94kDa/104kDa
Observed MW:93kDa
Synonyms:TNNI3K, CARK, CCDD
Background:This gene encodes a protein that belongs to the MAP kinase kinase kinase (MAPKKK) family of protein kinases. The protein contains ankyrin repeat, protein kinase and serine-rich domains and is thought to play a role in cardiac physiology.
UniProt Protein Function:May play a role in cardiac physiology.
NCBI Summary:This locus represents naturally occurring read-through transcription from the neighboring fucose-1-phosphate guanylyltransferase (FPGT) and TNNI3 interacting kinase (TNNI3K) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. [provided by RefSeq, Dec 2010]
UniProt Code:Q59H18
NCBI GenInfo Identifier:510785758
NCBI Gene ID:100526835
NCBI Accession:NP_001186256.2
UniProt Secondary Accession:Q59H18,Q17RN0, Q49AR1, Q6MZS9, Q9Y2V6,
Molecular Weight:104 kDa
NCBI Full Name:FPGT-TNNI3K fusion protein isoform b
NCBI Synonym Full Names:FPGT-TNNI3K readthrough
NCBI Official Symbol:FPGT-TNNI3K  
NCBI Official Synonym Symbols:CARK; TNNI3K  
NCBI Protein Information:FPGT-TNNI3K fusion protein
UniProt Protein Name:Serine/threonine-protein kinase TNNI3K
UniProt Synonym Protein Names:Cardiac ankyrin repeat kinase; Cardiac troponin I-interacting kinase; TNNI3-interacting kinase
UniProt Gene Name:TNNI3K  
Anti-TNNI3K Antibody (CAB7802)Western blot analysis of extracts of various cell lines, using TNNI3K antibody (CAB7802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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