ChIP Antibodies

Anti-TNNI3 Antibody (CAB6995)

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SKU:
CAB6995
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TNNI3 Antibody
Antibody SKU:CAB6995
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse heart, Rat heart, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADG SSDA AREP RPAP APIR RRSS NYRA YATE PHAK KKSK ISAS RKLQ LKTL LLQI AKQE LERE AEER RGEK GRAL STRC QPLE LAGL GFAE LQDL CRQL HARV DKVD EERY DIEA KVTK NITE IADL TQKI FDLR GKFK RPTL RRVR ISAD AMMQ ALLG ARAK ESLD LRAH LKQV KKED TEKE NREV GDWR KNID ALSG MEGR KKKF ES
Gene ID:7137
Uniprot:P19429
Cellular Location:
Calculated MW:24kDa
Observed MW:26kDa
Synonyms:TNNI3, CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I3
Background:Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
UniProt Protein Function:TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
UniProt Protein Details:

Protein type:Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cytosol; sarcomere; troponin complex

Molecular Function:actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1

NCBI Summary:Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
UniProt Code:P19429
NCBI GenInfo Identifier:136213
NCBI Gene ID:7137
NCBI Accession:P19429.3
UniProt Secondary Accession:P19429,P48787, P23693,
UniProt Related Accession:P19429
Molecular Weight:24,008 Da
NCBI Full Name:Troponin I, cardiac muscle
NCBI Synonym Full Names:troponin I3, cardiac type
NCBI Official Symbol:TNNI3  
NCBI Official Synonym Symbols:CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF  
NCBI Protein Information:troponin I, cardiac muscle
UniProt Protein Name:Troponin I, cardiac muscle
UniProt Synonym Protein Names:Cardiac troponin I
Protein Family:Troponin
UniProt Gene Name:TNNI3  
UniProt Entry Name:TNNI3_HUMAN
Anti-TNNI3 Antibody (CAB6995)Western blot analysis of extracts of various cell lines, using TNNI3 antibody (CAB6995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-TNNI3 Antibody (CAB6995)Immunofluorescence analysis of mouse heart cells using TNNI3 antibody (CAB6995) at dilution of 1:100. Blue: DAPI for nuclear staining.
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