Cell Biology Antibodies 9

Anti-TNNI1 Antibody (CAB4161)

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SKU:
CAB4161
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TNNI1 Antibody
Antibody SKU:CAB4161
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TNNI1 (NP_003272.3).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse skeletal muscle, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human TNNI1 (NP_003272.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPEV ERKP KITA SRKL LLKS LMLA KAKE CWEQ EHEE REAE KVRY LAER IPTL QTRG LSLS ALQD LCRE LHAK VEVV DEER YDIE AKCL HNTR EIKD LKLK
Gene ID:7135
Uniprot:P19237
Cellular Location:
Calculated MW:21kDa
Observed MW:22kDa
Synonyms:TNNI1, SSTNI, TNN1, troponin I1
Background:Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
UniProt Protein Function:TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: troponin complex; cytosol

Molecular Function:metal ion binding; actin binding

Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding

NCBI Summary:Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]
UniProt Code:P19237
NCBI GenInfo Identifier:1351298
NCBI Gene ID:7135
NCBI Accession:P19237.3
UniProt Secondary Accession:P19237,Q659A5, Q6FGS7, Q6FGW1, Q6ICU2, Q86T57, Q96DT9 A6NEH3, A8MSJ0,
UniProt Related Accession:P19237
Molecular Weight:187
NCBI Full Name:Troponin I, slow skeletal muscle
NCBI Synonym Full Names:troponin I type 1 (skeletal, slow)
NCBI Official Symbol:TNNI1  
NCBI Official Synonym Symbols:TNN1; SSTNI  
NCBI Protein Information:troponin I, slow skeletal muscle; troponin I, slow-twitch isoform
UniProt Protein Name:Troponin I, slow skeletal muscle
UniProt Synonym Protein Names:Troponin I, slow-twitch isoform
Protein Family:Troponin
UniProt Gene Name:TNNI1  
UniProt Entry Name:TNNI1_HUMAN
Anti-TNNI1 Antibody (CAB4161)Western blot analysis of extracts of various cell lines, using TNNI1 antibody (CAB4161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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