Cell Biology Antibodies 8
Anti-TNK2 Antibody (CAB2526)
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- SKU:
- CAB2526
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TNK2 Antibody |
| Antibody SKU: | CAB2526 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-385 of human TNK2 (NP_001010938.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | SW620, HT-29, MCF7, Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-385 of human TNK2 (NP_001010938.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TGWL LELL SEVQ LQQY FLRL RDDL NVTR LSHF EYVK NEDL EKIG MGRP GQRR LWEA VKRR KALC KRKS WMSK VFSG KRLE AEFP PHHS QSTF RKTS PAPG GPAG EGPL QSLT CLIG EKDL RLLE KLGD GSFG VVRR GEWD APSG KTVS VAVK CLKP DVLS QPEA MDDF IREV NAMH SLDH RNLI RLYG VVLT PPMK MVTE LAPL GSLL DRLR KHQG HFLL GTLS RYAV QVAE GMGY LESK RFIH RDLA ARNL LLAT RDLV KIGD FGLM RALP QNDD HYVM QEHR KVPF AWCA PESL KTRT FSHA SDTW MFGV TLWE |
| Gene ID: | 10188 |
| Uniprot: | Q07912 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endosome, Membrane, Nucleus, Peripheral membrane protein, adherens junction, clathrin-coated pit, clathrin-coated vesicle |
| Calculated MW: | 60kDa/114kDa/119kDa |
| Observed MW: | 115kDa |
| Synonyms: | TNK2, ACK, ACK-1, ACK1, p21cdc42Hs |
| Background: | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. |
| UniProt Protein Function: | Ack: a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential. Two differentially spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Ack family Chromosomal Location of Human Ortholog: 3q29 Cellular Component: clathrin-coated vesicle; coated pit; cytoplasm; endosome; extrinsic to internal side of plasma membrane; membrane; nucleus; plasma membrane Molecular Function:epidermal growth factor receptor binding; GTPase inhibitor activity; identical protein binding; protein binding; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; WW domain binding Biological Process: cell differentiation; cell migration; cell surface receptor linked signal transduction; innate immune response; phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell proliferation; small GTPase mediated signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway |
| NCBI Summary: | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q07912 |
| NCBI GenInfo Identifier: | 229462980 |
| NCBI Gene ID: | 10188 |
| NCBI Accession: | Q07912.3 |
| UniProt Secondary Accession: | Q07912,Q6ZMQ0, Q8N6U7, Q96H59, |
| UniProt Related Accession: | Q07912 |
| Molecular Weight: | 119,349 Da |
| NCBI Full Name: | Activated CDC42 kinase 1 |
| NCBI Synonym Full Names: | tyrosine kinase non receptor 2 |
| NCBI Official Symbol: | TNK2 |
| NCBI Official Synonym Symbols: | ACK; ACK1; ACK-1; p21cdc42Hs |
| NCBI Protein Information: | activated CDC42 kinase 1 |
| UniProt Protein Name: | Activated CDC42 kinase 1 |
| UniProt Synonym Protein Names: | Tyrosine kinase non-receptor protein 2 |
| Protein Family: | Activated CDC42 kinase |
| UniProt Gene Name: | TNK2 |
| UniProt Entry Name: | ACK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNK2 antibody (CAB2526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using TNK2 antibody (CAB2526). Blue: DAPI for nuclear staining. |
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