Antibody Name:	Anti-TNFRSF11A Antibody
Antibody SKU:	CAB13382
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 466-616 of human TNFRSF11A (NP_003830.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, HT-29, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 466-616 of human TNFRSF11A (NP_003830.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RGPL PQCA YGMG LPPE EEAS RTEA RDQP EDGA DGRL PSSA RAGA GSGS SPGG QSPA SGNV TGNS NSTF ISSG QVMN FKGD IIVV YVSQ TSQE GAAA AAEP MGRP VQEE TLAR RDSF AGNG PRFP DPCG GPEG LREP EKAS RPVQ EQGG AKA
Gene ID:	8792
Uniprot:	Q9Y6Q6
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	28kDa/32kDa/36kDa/56kDa/64kDa/66kDa
Observed MW:	90kDa

Synonyms:

TNFRSF11A, CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TRANCER

Background:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus.

UniProt Protein Function:	TNFRSF11A: Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts (via cytoplasmic domain) with GAB2. Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 18q21.33 Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane Molecular Function:cytokine binding; protein binding; receptor activity; transmembrane receptor activity; tumor necrosis factor receptor activity Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell-cell signaling; circadian thermoregulation; inflammatory response; osteoclast differentiation; positive regulation of cell proliferation; positive regulation of JNK activity; positive regulation of transcription factor activity; regulation of apoptosis; response to cytokine stimulus; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway Disease: Familial Expansile Osteolysis; Osteopetrosis, Autosomal Recessive 7; Paget Disease Of Bone
NCBI Summary:	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptors can interact with various TRAF family proteins, through which this receptor induces the activation of NF-kappa B and MAPK8/JNK. This receptor and its ligand are important regulators of the interaction between T cells and dendritic cells. This receptor is also an essential mediator for osteoclast and lymph node development. Mutations at this locus have been associated with familial expansile osteolysis, autosomal recessive osteopetrosis, and Paget disease of bone. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Aug 2012]
UniProt Code:	Q9Y6Q6
NCBI GenInfo Identifier:	19924309
NCBI Gene ID:	8792
NCBI Accession:	Q9Y6Q6.1
UniProt Secondary Accession:	Q9Y6Q6,Q59EP9, I4EC36, I4EC38, I4EC39, I7JE63, N0GVH0
UniProt Related Accession:	Q9Y6Q6
Molecular Weight:
NCBI Full Name:	Tumor necrosis factor receptor superfamily member 11A
NCBI Synonym Full Names:	TNF receptor superfamily member 11a
NCBI Official Symbol:	TNFRSF11A
NCBI Official Synonym Symbols:	FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1
NCBI Protein Information:	tumor necrosis factor receptor superfamily member 11A
UniProt Protein Name:	Tumor necrosis factor receptor superfamily member 11A
UniProt Synonym Protein Names:	Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; CD_antigen: CD265
UniProt Gene Name:	TNFRSF11A

	Western blot analysis of extracts of various cell lines, using TNFRSF11A antibody (CAB13382) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse brain using TNFRSF11A antibody (CAB13382) at dilution of 1:100 (20x lens).
	Immunofluorescence analysis of L929 cells using TNFRSF11A Polyclonal Antibody (CAB13382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.