KO Validated Antibodies 1

Anti-TNFAIP3 Antibody (CAB18056)[KO Validated]

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SKU:
CAB18056
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-TNFAIP3 Antibody [KO Validated]
Antibody SKU:CAB18056
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human TNFAIP3 (NP_006281.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human TNFAIP3 (NP_006281.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEQ VLPQ ALYL SNMR KAVK IRER TPED IFKP TNGI IHHF KTMH RYTL EMFR TCQF CPQF REII HKAL IDRN IQAT LESQ KKLN WCRE VRKL VALK TNGD GNCL MHAT SQYM WGVQ DTDL VLRK ALFS TLKE TDTR NFKF RWQL ESLK SQEF VETG LCYD TRNW NDEW DNLI KMAS TDTP MARS GLQY NS
Gene ID:7128
Uniprot:P21580
Cellular Location:Cytoplasm, Lysosome, Nucleus
Calculated MW:89kDa
Observed MW:80kDa
Anti-TNFAIP3 Antibody [KO Validated] (CAB18056)Western blot analysis of extracts from normal (control) and TNFAIP3 knockout (KO) A-549 cells, using TNFAIP3 antibody (CAB18056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:A20, AISBL, OTUD7C, TNFA1P2, TNFAIP3
Background:This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.
UniProt Protein Details:

Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Apoptosis; Protease

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: centrosome; lysosome; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; ubiquitin binding; DNA binding; protease binding; protein self-association; zinc ion binding; ubiquitin-specific protease activity; ubiquitin-protein ligase activity; kinase binding; ligase activity

Biological Process: negative regulation of toll-like receptor 5 signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; negative regulation of interleukin-6 production; negative regulation of bone resorption; inflammatory response; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of toll-like receptor 2 signaling pathway; B-1 B cell homeostasis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of B cell activation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; protein oligomerization; regulation of germinal center formation; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of protein catabolic process; innate immune response; negative regulation of protein ubiquitination; regulation of defense response to virus by host; negative regulation of interferon type I production

NCBI Summary:This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:P21580
NCBI GenInfo Identifier:112894
NCBI Gene ID:7128
NCBI Accession:P21580.1
UniProt Secondary Accession:P21580,Q2HIX9, Q5VXQ7, Q9NSR6, B2R767, E1P588,
UniProt Related Accession:P21580
Molecular Weight:790
NCBI Full Name:Tumor necrosis factor alpha-induced protein 3
NCBI Synonym Full Names:tumor necrosis factor, alpha-induced protein 3
NCBI Official Symbol:TNFAIP3  
NCBI Official Synonym Symbols:A20; OTUD7C; TNFA1P2  
NCBI Protein Information:tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20
UniProt Protein Name:Tumor necrosis factor alpha-induced protein 3
UniProt Synonym Protein Names:OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20Cleaved into the following 2 chains:A20p50; A20p37
Protein Family:TNFAIP3-interacting protein
UniProt Gene Name:TNFAIP3  
UniProt Entry Name:TNAP3_HUMAN
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