Name: Anti-TNF Antibody (MACO0517)
SKU: MACO0517
Availability: OutOfStock

Product Name:	Anti-TNF Antibody
Product Sku:	MACO0517
Size:	50ug
Host Species:	Mouse
Tested Applications:	ELISA
Recommended Dilutions:
Species Reactivity:	Bovine
Immunogen:	Recombinant Bovine TNF alpha protein

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%,Protein G purified
Clonality:	Monoclonal
Isotype:	IgG2b
Conjugate:	Non-conjugated
Background:	Tumor necrosis factor alpha (TNF?,also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF? is produced by a wide variety of immune and epithelial cell types. Bovine TNF? consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD). Within the ECD, bovine TNF? shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNF?.The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF? by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF?. TNF? trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNF? regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF? is a key cytokine in several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
Synonyms:	Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2

UniProt Protein Function:	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q06599
NCBI GenInfo Identifier:	150421693
NCBI Gene ID:	280943
NCBI Accession:	Q06599.2
UniProt Secondary Accession:	Q06599,O18779, Q27978, A4IFT5, A9QWR7
UniProt Related Accession:	Q06599
Molecular Weight:	25,439 Da
NCBI Full Name:	Tumor necrosis factor
NCBI Synonym Full Names:
NCBI Official Symbol:	TNF
NCBI Official Synonym Symbols:	TNFa; TNF-a; TNF-alpha
NCBI Protein Information:	tumor necrosis factor
UniProt Protein Name:	Tumor necrosis factor
UniProt Synonym Protein Names:	Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Protein Family:
UniProt Gene Name:	TNF
UniProt Entry Name:	TNFA_BOVIN