Signal Transduction Antibodies 1
Anti-TMEM27 Antibody (CAB12715)
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- SKU:
- CAB12715
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-TMEM27 Antibody |
| Antibody SKU: | CAB12715 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human TMEM27 (NP_065716.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse pancreas, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human TMEM27 (NP_065716.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLWL LFFL VTAI HAEL CQPG AENA FKVR LSIR TALG DKAY AWDT NEEY LFKA MVAF SMRK VPNR EATE ISHV LLCN VTQR VSFW FVVT DPSK NHTL PAVE VQSA IRMN KNRI NNAF FLND QTLE FLKI PSTL APPM DPSV PIWI IIFG VIFC IIIV AIAL LILS GIWQ RRRK NKEP SEVD DAED KCEN MITI ENGI PSDP LDMK GGHI NDAF MTED ERLT PL |
| Gene ID: | 57393 |
| Uniprot: | Q9HBJ8 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 25kDa |
| Observed MW: | 29kDa |
| Synonyms: | TMEM27, NX-17, NX17 |
| Background: | This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. |
| UniProt Protein Function: | TMEM27: Regulator of SNARE complex function. Stimulator of beta cell replication. Belongs to the TMEM27 family. |
| UniProt Protein Details: | Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: Xp22 Cellular Component: brush border membrane; cytoplasm; integral to membrane Molecular Function:peptidyl-dipeptidase activity; protein binding; metallopeptidase activity Biological Process: positive regulation of calcium ion-dependent exocytosis; proteolysis; positive regulation of amino acid transport |
| NCBI Summary: | This gene encodes a type 1 transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. The encoded protein binds to amino acid transporters and regulates their expression on the plasma membrane. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. The extracellular domain of the encoded protein may be cleaved and shed from the plasma membrane specifically in pancreatic beta cells. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q9HBJ8 |
| NCBI GenInfo Identifier: | 74734254 |
| NCBI Gene ID: | 57393 |
| NCBI Accession: | Q9HBJ8.1 |
| UniProt Secondary Accession: | Q9HBJ8,Q6UW07, B2R9M1, |
| UniProt Related Accession: | Q9HBJ8 |
| Molecular Weight: | 222 |
| NCBI Full Name: | Collectrin |
| NCBI Synonym Full Names: | transmembrane protein 27 |
| NCBI Official Symbol: | TMEM27 |
| NCBI Official Synonym Symbols: | NX17; NX-17 |
| NCBI Protein Information: | collectrin; kidney-specific membrane protein |
| UniProt Protein Name: | Collectrin |
| UniProt Synonym Protein Names: | Transmembrane protein 27 |
| Protein Family: | Collectrin |
| UniProt Gene Name: | TMEM27 |
| UniProt Entry Name: | TMM27_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TMEM27 antibody (CAB12715) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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