Cell Biology Antibodies 4
Anti-TMEM25 Antibody (CAB14370)
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- SKU:
- CAB14370
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TMEM25 Antibody |
| Antibody SKU: | CAB14370 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 223-322 of human TMEM25 (NP_001137506.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, OVCAR3, LO2, Mouse brain, Mouse kidney, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 223-322 of human TMEM25 (NP_001137506.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APGP SRHP SLIS SDSN NLKL NNVR LPRE NMSL PSNL QLND LTPD SRAV KPAD RQMA QNNS RPEL LDPE PGGL LTSQ GFIR LPVL GYIY RVSS VSSD EIWL |
| Gene ID: | 84866 |
| Uniprot: | Q86YD3 |
| Cellular Location: | Cell membrane, Secreted, Single-pass type I membrane protein |
| Calculated MW: | 23kDa/33kDa/34kDa/37kDa/39kDa |
| Observed MW: | 50kDa |
| Synonyms: | TMEM25 |
| Background: |
| UniProt Protein Function: | TMEM25: 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23.3 Molecular Function:protein binding |
| UniProt Code: | Q86YD3 |
| NCBI GenInfo Identifier: | 221139830 |
| NCBI Gene ID: | 84866 |
| NCBI Accession: | NP_001137506.1 |
| UniProt Secondary Accession: | Q86YD3,Q6UW89, Q86UA7, Q8NBL5, Q96KA6, Q96MW9, A8K8J4 B0YJA6, B0YJA7, B0YJA9, G5E9U4, |
| Molecular Weight: | 48.2kD |
| NCBI Full Name: | transmembrane protein 25 isoform 2 |
| UniProt Protein Name: | Transmembrane protein 25 |
| Protein Family: | Transmembrane protein |
| UniProt Gene Name: | TMEM25 |
 | Western blot analysis of extracts of various cell lines, using TMEM25 antibody (CAB14370) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using TMEM25 antibody (CAB14370) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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