Metabolism Antibodies 3

Anti-TMEM189 Antibody (CAB8380)

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SKU:
CAB8380
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-TMEM189 Antibody
Antibody SKU:CAB8380
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human TMEM189 (NP_954580.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human
Positive Samples:MCF7
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human TMEM189 (NP_954580.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TLLQ DWHV ILPR KHHR IHHV SPHE TYFC ITTG WLNY PLEK IGFW RRLE DLIQ GLTG EKPR ADDM KWAQ KIK
Gene ID:387521
Uniprot:A5PLL7
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:30kDa/31kDa
Observed MW:31kDa
Synonyms:TMEM189, KUA
Background:Co-transcription of this gene and the neighboring downstream gene (ubiquitin-conjugating enzyme E2 variant 1) generates a rare read-through transcript, which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. The protein encoded by this individual gene lacks a UEV1 domain but includes three transmembrane regions. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:TMEM189: 2 isoforms of the human protein are produced by alternative splicing
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: cytoplasm; endoplasmic reticulum membrane; integral to membrane

Molecular Function:ubiquitin protein ligase binding

Biological Process: protein ubiquitination

NCBI Summary:Co-transcription of this gene and the neighboring downstream gene (ubiquitin-conjugating enzyme E2 variant 1) generates a rare read-through transcript, which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. The protein encoded by this individual gene lacks a UEV1 domain but includes three transmembrane regions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
UniProt Code:A5PLL7
NCBI GenInfo Identifier:241982751
NCBI Gene ID:387521
NCBI Accession:NP_001155977.1
UniProt Related Accession:A5PLL7
Molecular Weight:30,851 Da
NCBI Full Name:transmembrane protein 189 isoform 2
NCBI Synonym Full Names:transmembrane protein 189
NCBI Official Symbol:TMEM189  
NCBI Official Synonym Symbols:KUA  
NCBI Protein Information:transmembrane protein 189
UniProt Protein Name:Transmembrane protein 189
Protein Family:Transmembrane protein
UniProt Gene Name:TMEM189  
UniProt Entry Name:TM189_HUMAN
Anti-TMEM189 Polyclonal Antibody (CAB8380)Western blot analysis of extracts of MCF-7 cells, using TMEM189 antibody (CAB8380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TMEM189 Polyclonal Antibody (CAB8380)Immunohistochemistry of paraffin-embedded human liver using TMEM189 antibody (CAB8380) at dilution of 1:100 (40x lens).
Anti-TMEM189 Polyclonal Antibody (CAB8380)Immunohistochemistry of paraffin-embedded human stomach using TMEM189 antibody (CAB8380) at dilution of 1:100 (40x lens).
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