Cell Biology Antibodies 8
Anti-TMED9 Antibody (CAB3442)
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- SKU:
- CAB3442
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TMED9 Antibody |
| Antibody SKU: | CAB3442 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human TMED9 (NP_059980.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, HeLa, Raji, Mouse liver, Mouse brain, Mouse lung, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human TMED9 (NP_059980.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAVE LGVL LVRP RPGT GLGR VMRT LLLV LWLA TRGS ALYF HIGE TEKK CFIE EIPD ETMV IGNY RTQL YDKQ REEY QPAT PGLG MFVE VKDP EDKV ILAR QYGS EGRF TFTS HTPG EHQI CLHS NSTK FSLF AGGM LRVH LDIQ VGEH ANDY AEIA AKDK LSEL QLRV RQLV EQVE QIQK EQNY QRWR EERF RQTS ESTN QRVL WWSI LQTL ILVA IGVW QMRH |
| Gene ID: | 54732 |
| Uniprot: | Q9BVK6 |
| Cellular Location: | Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus, Single-pass type I membrane protein, cis-Golgi network membrane, trans-Golgi network membrane |
| Calculated MW: | 27kDa |
| Observed MW: | 27kDa |
| Synonyms: | TMED9, GMP25, HSGP25L2G, p24a2, p24alpha2, p25 |
| Background: |
| UniProt Protein Function: | TMED9: Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer- dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB. Belongs to the EMP24/GP25L family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; ER-Golgi intermediate compartment; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; integral to membrane; trans-Golgi network transport vesicle; transport vesicle Molecular Function:protein binding; syntaxin binding Biological Process: cellular protein metabolic process; COPI coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein exit from endoplasmic reticulum |
| UniProt Code: | Q9BVK6 |
| NCBI GenInfo Identifier: | 239938724 |
| NCBI Gene ID: | 54732 |
| NCBI Accession: | Q9BVK6.2 |
| UniProt Secondary Accession: | Q9BVK6,Q14437, Q8WZ61, |
| UniProt Related Accession: | Q9BVK6 |
| Molecular Weight: | 27,277 Da |
| NCBI Full Name: | Transmembrane emp24 domain-containing protein 9 |
| NCBI Synonym Full Names: | transmembrane p24 trafficking protein 9 |
| NCBI Official Symbol: | TMED9 |
| NCBI Official Synonym Symbols: | p25; GMP25; HSGP25L2G |
| NCBI Protein Information: | transmembrane emp24 domain-containing protein 9 |
| UniProt Protein Name: | Transmembrane emp24 domain-containing protein 9 |
| UniProt Synonym Protein Names: | GMP25; Glycoprotein 25L2; p24 family protein alpha-2; p24alpha2; p25 |
| Protein Family: | Transmembrane emp24 domain-containing protein |
| UniProt Gene Name: | TMED9 |
| UniProt Entry Name: | TMED9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TMED9 antibody (CAB3442) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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