Immunology Antibodies 1
Anti-TLR3 Antibody (CAB11778)
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- SKU:
- CAB11778
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TLR3 Antibody |
| Antibody SKU: | CAB11778 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 464-647 of human TLR3 (NP_003256.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, LO2, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 464-647 of human TLR3 (NP_003256.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SYNK YLQL TRNS FALV PSLQ RLML RRVA LKNV DSSP SPFQ PLRN LTIL DLSN NNIA NIND DMLE GLEK LEIL DLQH NNLA RLWK HANP GGPI YFLK GLSH LHIL NLES NGFD EIPV EVFK DLFE LKII DLGL NNLN TLPA SVFN NQVS LKSL NLQK NLIT SVEK KVFG PAFR NLTE LDMR FNPF |
| Gene ID: | 7098 |
| Uniprot: | O15455 |
| Cellular Location: | Early endosome, Endoplasmic reticulum membrane, Endosome membrane, Single-pass type I membrane protein |
| Calculated MW: | 72kDa/103kDa |
| Observed MW: | 120kDa |
| Synonyms: | TLR3, CD283, IIAE2 |
| Background: | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. |
| UniProt Code: | O15455 |
| NCBI GenInfo Identifier: | 20140422 |
| NCBI Gene ID: | 7098 |
| NCBI Accession: | O15455.1 |
| UniProt Secondary Accession: | O15455,Q4VAL2, Q504W0, B2RAI7, B7Z7K0, E6Y0F0, E6Y0F1 E9PGH4, |
| UniProt Related Accession: | O15455 |
| Molecular Weight: | 72,937 Da |
| NCBI Full Name: | Toll-like receptor 3 |
| NCBI Synonym Full Names: | toll like receptor 3 |
| NCBI Official Symbol: | TLR3 |
| NCBI Official Synonym Symbols: | CD283; IIAE2 |
| NCBI Protein Information: | toll-like receptor 3 |
| UniProt Protein Name: | Toll-like receptor 3 |
| UniProt Synonym Protein Names: | CD_antigen: CD283 |
| Protein Family: | Toll-like receptor |
| UniProt Gene Name: | TLR3 |
 | Western blot analysis of extracts of various cell lines, using TLR3 antibody (CAB11778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of various cell lines, using TLR3 antibody (CAB11778) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human placenta using TLR3 Rabbit pAb (CAB11778) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using TLR3 antibody (CAB11778) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using TLR3 antibody (CAB11778) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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