Antibody Name:	Anti-TLN1 Antibody
Antibody SKU:	CAB4158
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLN1 (NP_006280.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLN1 (NP_006280.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVAL SLKI SIGN VVKT MQFE PSTM VYDA CRII RERI PEAP AGPP SDFG LFLS DDDP KKGI WLEA GKAL DYYM LRNG DTME YRKK QRPL KIRM LDGT VKTI MVDD SKTV TDML MTIC ARIG ITNH DEYS LVRE LMEE KKEE ITGT LRKD KTLL RDEK KMEK LKQK LHTD DELN WLDH GRTL REQG VEEH ETLL LRRK FFYS DQNV DSRD PVQL NLLY VQAR
Gene ID:	7094
Uniprot:	Q9Y490
Cellular Location:	Cell junction, Cell projection, Cell surface, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, focal adhesion, ruffle membrane
Calculated MW:	269kDa
Observed MW:	240kDa

Synonyms:

TLN1, ILWEQ, TLN, talin-1

Background:

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

UniProt Protein Function:	Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 9p13 Cellular Component: ruffle; cell surface; focal adhesion; cytoplasm; plasma membrane; extracellular region; microtubule organizing center; intercellular junction; cytosol Molecular Function:integrin binding; actin filament binding; LIM domain binding; protein binding; structural constituent of cytoskeleton; insulin receptor binding; vinculin binding Biological Process: platelet activation; intercellular junction assembly; axon guidance; unfolded protein response; cytoskeletal anchoring; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; platelet degranulation; muscle contraction; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cell motility; blood coagulation
NCBI Summary:	This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009]
UniProt Code:	Q9Y490
NCBI GenInfo Identifier:	81175200
NCBI Gene ID:	7094
NCBI Accession:	Q9Y490.3
UniProt Secondary Accession:	Q9Y490,Q86YD0, Q9NZQ2, Q9UHH8, Q9UPX3, A6NMY0,
UniProt Related Accession:	Q9Y490
Molecular Weight:	2541
NCBI Full Name:	Talin-1
NCBI Synonym Full Names:	talin 1
NCBI Official Symbol:	TLN1
NCBI Official Synonym Symbols:	TLN; ILWEQ
NCBI Protein Information:	talin-1
UniProt Protein Name:	Talin-1
Protein Family:	Talin
UniProt Gene Name:	TLN1
UniProt Entry Name:	TLN1_HUMAN

	Western blot analysis of extracts of U-87MG cells, using TLN1 antibody (CAB4158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunofluorescence analysis of rat Intestine using TLN1 antibody (CAB4158) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of human colon carcinoma using TLN1 antibody (CAB4158) at dilution of 1:100. Blue: DAPI for nuclear staining.