Antibody Name:	Anti-TLK2 Antibody [KO Validated]
Antibody SKU:	CAB6770
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	293T, Mouse testis, NIH/3T3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TLK2 (NP_006843.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMEE LHSL DPRR QELL EARF TGVG VSKG PLNS ESSN QSLC SVGS LSDK EVET PEKK QNDQ RNRK RKAE PYET SQGK GTPR GHKI SDYF EFAG GSAP GTSP GRSV PPVA RSSP QHSL SNPL PRRV EQPL YGLD GSAA KEAT EEQS ALPT LMSV MLAK PRLD TEQL AQRG AGLC FTFV SAQQ NSPS STGS GNTE HSCS SQKQ ISIQ HRQT QSDL TIEK ISAL
Gene ID:	11011
Uniprot:	Q86UE8
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, perinuclear region
Calculated MW:	82kDa/85kDa/87kDa
Observed MW:	87kDa

Synonyms:

TLK2, HsHPK, PKU-ALPHA

Background:

This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	TLK2: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; TLK family Chromosomal Location of Human Ortholog: 17q23 Cellular Component: cell junction; intermediate filament; nucleus; perinuclear region of cytoplasm Molecular Function:ATP binding; protein binding; protein serine/threonine kinase activity Biological Process: chromosome segregation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; peptidyl-serine phosphorylation; protein amino acid phosphorylation; regulation of chromatin assembly or disassembly; response to DNA damage stimulus
NCBI Summary:	This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	Q86UE8
NCBI GenInfo Identifier:	34222826
NCBI Gene ID:	11011
NCBI Accession:	Q86UE8.2
UniProt Secondary Accession:	Q86UE8,Q9UKI7, Q9Y4F7, D3DU07,
UniProt Related Accession:	Q86UE8
Molecular Weight:	82,348 Da
NCBI Full Name:	Serine/threonine-protein kinase tousled-like 2
NCBI Synonym Full Names:	tousled like kinase 2
NCBI Official Symbol:	TLK2
NCBI Official Synonym Symbols:	HsHPK; PKU-ALPHA
NCBI Protein Information:	serine/threonine-protein kinase tousled-like 2
UniProt Protein Name:	Serine/threonine-protein kinase tousled-like 2
UniProt Synonym Protein Names:	HsHPK; PKU-alpha; Tousled-like kinase 2
Protein Family:	Serine/threonine-protein kinase tousled
UniProt Gene Name:	TLK2
UniProt Entry Name:	TLK2_HUMAN

	Western blot analysis of extracts of various cell lines, using TLK2 antibody (CAB6770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and TLK2 Rabbit pAb knockout (KO) NIH/3T3 cells, using TLK2 Rabbit pAb antibody (CAB6770) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human liver damage using TLK2 antibody (CAB6770) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using TLK2 antibody (CAB6770) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TLK2 antibody (CAB6770). Blue: DAPI for nuclear staining.