Cell Cycle Antibodies 1
Anti-TLK1 Antibody (CAB14831)
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- SKU:
- CAB14831
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-TLK1 Antibody |
| Antibody SKU: | CAB14831 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-230 of human TLK1 (NP_036422.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, 293T, BxPC-3, HeLa, Jurkat, Mouse testis, Mouse pancreas, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-230 of human TLK1 (NP_036422.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PVRG IPPA IRSP QNSH SHST PSSS VRPN SPSP TALA FGDH PIVQ PKQL SFKI IQTD LTML KLAA LESN KIQ |
| Gene ID: | 9874 |
| Uniprot: | Q9UKI8 |
| Cellular Location: | Nucleus |
| Calculated MW: | 63kDa/77kDa/81kDa/86kDa/89kDa |
| Observed MW: | 80kDa |
| Synonyms: | TLK1, PKU-beta |
| Background: | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'. |
| NCBI Summary: | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9UKI8 |
| NCBI GenInfo Identifier: | 34223086 |
| NCBI Gene ID: | 9874 |
| NCBI Accession: | Q9UKI8.2 |
| UniProt Secondary Accession: | Q9UKI8,Q14150, Q8N591, Q9NYH2, Q9Y4F6, B3KR15, B4DX87 |
| UniProt Related Accession: | Q9UKI8 |
| Molecular Weight: | 81,927 Da |
| NCBI Full Name: | Serine/threonine-protein kinase tousled-like 1 |
| NCBI Synonym Full Names: | tousled like kinase 1 |
| NCBI Official Symbol: | TLK1 |
| NCBI Official Synonym Symbols: | PKU-beta |
| NCBI Protein Information: | serine/threonine-protein kinase tousled-like 1 |
| UniProt Protein Name: | Serine/threonine-protein kinase tousled-like 1 |
| UniProt Synonym Protein Names: | PKU-beta; Tousled-like kinase 1 |
| Protein Family: | Serine/threonine-protein kinase tousled |
| UniProt Gene Name: | TLK1 |
| UniProt Entry Name: | TLK1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TLK1 antibody (CAB14831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat heart using TLK1 antibody (CAB14831) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using TLK1 antibody (CAB14831) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using TLK1 antibody (CAB14831) at dilution of 1:100 (40x lens). |
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