Cell Biology Antibodies 10
Anti-TKT Antibody (CAB6314)
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- SKU:
- CAB6314
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TKT Antibody |
| Antibody SKU: | CAB6314 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TKT (NP_001128527.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, 293T, Jurkat, MCF7, U-251MG, HepG2, Mouse liver, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TKT (NP_001128527.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MESY HKPD QQKL QALK DTAN RLRI SSIQ ATTA AGSG HPTS CCSA AEIM AVLF FHTM RYKS QDPR NPHN DRFV LSKG HAAP ILYA VWAE AGFL AEAE LLNL RKIS SDLD GHPV PKQA FTDV ATGS LGQG LGAA CGMA YTGK YFDK ASYR VYCL LGDG ELSE GSVW EAMA FASI YKLD NLVA ILDI NRLG QSDP APLQ HQMD IYQK RCEA FGWH AIIV DGHS VEEL CKAF GQAK HQPT AIIA KTFK GRGI TGVE DKES WHGK PLPK NMAE QIIQ EIYS QIQS |
| Gene ID: | 7086 |
| Uniprot: | P29401 |
| Cellular Location: | |
| Calculated MW: | 67kDa/68kDa |
| Observed MW: | 68kDa |
| Synonyms: | TKT, HEL-S-48, HEL107, SDDHD, TK, TKT1 |
| Background: | This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.2.1.1; Carbohydrate Metabolism - pentose phosphate pathway Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: nucleoplasm; peroxisome; nucleus; cytosol; vesicle Molecular Function:transketolase activity; protein homodimerization activity; metal ion binding; cofactor binding Biological Process: pentose-phosphate shunt; regulation of growth; carbohydrate metabolic process; energy reserve metabolic process; glyceraldehyde-3-phosphate biosynthetic process; pentose-phosphate shunt, non-oxidative branch; xylulose biosynthetic process; pathogenesis Disease: Wernicke-korsakoff Syndrome |
| NCBI Summary: | This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P29401 |
| NCBI GenInfo Identifier: | 1729976 |
| NCBI Gene ID: | 7086 |
| NCBI Accession: | P29401.3 |
| UniProt Secondary Accession: | P29401,Q8TBA3, Q96HH3, A8K089, B4DE31, E7EPA7, |
| UniProt Related Accession: | P29401 |
| Molecular Weight: | 623 |
| NCBI Full Name: | Transketolase |
| NCBI Synonym Full Names: | transketolase |
| NCBI Official Symbol: | TKT |
| NCBI Official Synonym Symbols: | TK; TKT1; HEL107 |
| NCBI Protein Information: | transketolase; epididymis luminal protein 107 |
| UniProt Protein Name: | Transketolase |
| Protein Family: | Transketolase |
| UniProt Gene Name: | TKT |
| UniProt Entry Name: | TKT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TKT antibody (CAB6314) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded Rat liver using TKT Rabbit pAb (CAB6314) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human esophageal using TKT Rabbit pAb (CAB6314) at dilution of 1:50 (40x lens). |
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