Epigenetics & Nuclear Signaling Antibodies 2
Anti-TK1 Antibody (CAB5612)
- SKU:
- CAB5612
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TK1 Antibody |
| Antibody SKU: | CAB5612 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human TK1 (NP_003249.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse spleen, Mouse ovary |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human TK1 (NP_003249.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSCI NLPT VLPG SPSK TRGQ IQVI LGPM FSGK STEL MRRV RRFQ IAQY KCLV IKYA KDTR YSSS FCTH DRNT MEAL PACL LRDV AQEA LGVA VIGI DEGQ FFPD IVEF CEAM ANAG KTVI VAAL DGTF QRKP FGAI LNLV PLAE SVVK LTAV CMEC FREA AYTK RLGT EKEV EVIG GADK YHSV CRLC YFKK ASGQ PAGP DNKE NCPV PGKP GEAV AARK LFAP QQIL QCSP AN |
| Gene ID: | 7083 |
| Uniprot: | P04183 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 25kDa |
| Observed MW: | 30kDa |
| Synonyms: | TK1, TK2 |
| Background: | The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. |
| UniProt Protein Function: | TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. |
| UniProt Protein Details: | Protein type:EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other Chromosomal Location of Human Ortholog: 17q23.2-q25.3 Cellular Component: cytosol Molecular Function:identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation |
| UniProt Code: | P04183 |
| NCBI GenInfo Identifier: | 23503074 |
| NCBI Gene ID: | 7083 |
| NCBI Accession: | P04183.2 |
| UniProt Secondary Accession: | P04183,Q969V0, Q9UMG9, B2RC58, |
| UniProt Related Accession: | P04183 |
| Molecular Weight: | 234 |
| NCBI Full Name: | Thymidine kinase, cytosolic |
| NCBI Synonym Full Names: | thymidine kinase 1, soluble |
| NCBI Official Symbol: | TK1 |
| NCBI Official Synonym Symbols: | TK2 |
| NCBI Protein Information: | thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform |
| UniProt Protein Name: | Thymidine kinase, cytosolic |
| Protein Family: | Thymidine kinase |
| UniProt Gene Name: | TK1 |
| UniProt Entry Name: | KITH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TK1 antibody (CAB5612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded Rat liver using TK1 antibody (CAB5612) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human esophageal using TK1 antibody (CAB5612) at dilution of 1:100 (40x lens). |
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