Cell Biology Antibodies 3
Anti-TJP3 Antibody (CAB13085)
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- SKU:
- CAB13085
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TJP3 Antibody |
| Antibody SKU: | CAB13085 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 710-919 of human TJP3 (NP_001254489.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 710-919 of human TJP3 (NP_001254489.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RQWL APAS RRST RRLY AQAQ KLRK HSSH LFTA TIPL NGTS DTWY QELK AIIR EQQT RPIW TAED QLDG SLED NLDL PHHG LADS SADL SCDS RVNS DYET DGEG GAYT DGEG YTDG EGGP YTDV DDEP PAPA LARS SEPV QADE SQSP RDRG RISA HQGA QVDS RHPQ GQWR QDSM RTYE REAL KKKF MRVH DAES SDED GYDW GPAT DL |
| Gene ID: | 27134 |
| Uniprot: | O95049 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, tight junction |
| Calculated MW: | 97kDa/101kDa/102kDa/103kDa |
| Observed MW: | 150kDa |
| Synonyms: | TJP3, ZO-3, ZO3 |
| Background: | The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. |
| UniProt Protein Function: | ZO3: Interacts with occludin, claudins and ZO-1. Interacts with INADL. Interacts with UBN1. Interacts with FASLG. Belongs to the MAGUK family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; tight junction; apical plasma membrane; cell junction; nucleus Molecular Function:protein binding |
| NCBI Summary: | The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012] |
| UniProt Code: | O95049 |
| NCBI GenInfo Identifier: | 597439632 |
| NCBI Gene ID: | 27134 |
| NCBI Accession: | O95049.3 |
| UniProt Related Accession: | O95049 |
| Molecular Weight: | |
| NCBI Full Name: | Tight junction protein ZO-3 |
| NCBI Synonym Full Names: | tight junction protein 3 |
| NCBI Official Symbol: | TJP3 |
| NCBI Official Synonym Symbols: | ZO3; ZO-3 |
| NCBI Protein Information: | tight junction protein ZO-3 |
| UniProt Protein Name: | Tight junction protein ZO-3 |
| UniProt Synonym Protein Names: | Tight junction protein 3; Zona occludens protein 3; Zonula occludens protein 3 |
| UniProt Gene Name: | TJP3 |
| UniProt Entry Name: | ZO3_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using TJP3 antibody (CAB13085) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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