Cell Biology Antibodies 10
Anti-TIMP4 Antibody (CAB6416)
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- SKU:
- CAB6416
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TIMP4 Antibody |
| Antibody SKU: | CAB6416 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_003247.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, Mouse heart, Mouse brain, Mouse ovary, Rat heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_003247.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CSCA PAHP QQHI CHSA LVIR AKIS SEKV VPAS ADPA DTEK MLRY EIKQ IKMF KGFE KVKD VQYI YTPF DSSL CGVK LEAN SQKQ YLLT GQVL SDGK VFIH LCNY IEPW EDLS LVQR ESLN HHYH LNCG CQIT TCYT VPCT ISAP NECL WTDW LLER KLYG YQAQ HYVC MKHV DGTC SWYR GHLP LRKE FVDI VQP |
| Gene ID: | 7079 |
| Uniprot: | Q99727 |
| Cellular Location: | Secreted |
| Calculated MW: | 25kDa |
| Observed MW: | 25kDa |
| Synonyms: | TIMP4 |
| Background: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. |
| UniProt Protein Function: | TIMP4: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP- 9. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p25 Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere Molecular Function:metal ion binding; metalloendopeptidase inhibitor activity; protease binding Biological Process: central nervous system development; negative regulation of membrane protein ectodomain proteolysis; negative regulation of metalloenzyme activity; Notch signaling pathway; ovulation cycle; response to cytokine stimulus; response to drug; response to lipopolysaccharide; response to organic substance; response to peptide hormone stimulus |
| NCBI Summary: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99727 |
| NCBI GenInfo Identifier: | 3915135 |
| NCBI Gene ID: | 7079 |
| NCBI Accession: | Q99727.1 |
| UniProt Secondary Accession: | Q99727,B2R7K6, |
| UniProt Related Accession: | Q99727 |
| Molecular Weight: | 25,503 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 4 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 4 |
| NCBI Official Symbol: | TIMP4 |
| NCBI Protein Information: | metalloproteinase inhibitor 4 |
| UniProt Protein Name: | Metalloproteinase inhibitor 4 |
| UniProt Synonym Protein Names: | Tissue inhibitor of metalloproteinases 4; TIMP-4 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP4 |
| UniProt Entry Name: | TIMP4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIMP4 antibody (CAB6416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using TIMP4 antibody (CAB6416). Blue: DAPI for nuclear staining. |
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