Antibody Name:	Anti-TIMM8B Antibody
Antibody SKU:	CAB15814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-98 of human TIMM8B (NP_036591.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, A-549, MCF7, Mouse brain, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 16-98 of human TIMM8B (NP_036591.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEL GEAD EAEL QRLV AAEQ QKAQ FTAQ VHHF MELC WDKC VEKP GNRL DSRT ENCL SSCV DRFI DTTL AITS RFAQ IVQK GGQ
Gene ID:	26521
Uniprot:	Q9Y5J9
Cellular Location:	Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	9kDa
Observed MW:	12kDa

Synonyms:

TIMM8B, DDP2, TIM8B

Background:

This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.

UniProt Protein Function:	Function: Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space
UniProt Protein Details:	By similarity. Subunit structure: Heterohexamer; possibly composed of 3 copies of TIMM8B and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 By similarity. Subcellular location: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity. Tissue specificity: Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle. Ref.2 Domain: The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8B from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity. Sequence similarities: Belongs to the small Tim family.
NCBI Summary:	This gene encodes a member of a well-conserved family of proteins with similarity to yeast Tim mitochondrial import proteins. This gene is encoded by a nuclear gene and is transported into the intermembrane space of the mitochondrion. When formed into complexes, these proteins guide membrane-spanning proteins across the mitochondrial intermembrane space before they are added into the mitochondrial inner membrane. This gene is adjacent to succinate dehydrogenase, subunit D (SDHD), in which mutations have been found in affected members of families with hereditary paraganglioma.[provided by RefSeq, Aug 2009]
UniProt Code:	Q9Y5J9
NCBI GenInfo Identifier:	6524627
NCBI Gene ID:	26521
NCBI Accession:	AAF15100.1
UniProt Secondary Accession:	Q9Y5J9,P62077, P62078,
UniProt Related Accession:	Q9Y5J9
Molecular Weight:	9,344 Da
NCBI Full Name:	TIMM8b
NCBI Synonym Full Names:	translocase of inner mitochondrial membrane 8 homolog B (yeast)
NCBI Official Symbol:	TIMM8B
NCBI Official Synonym Symbols:	DDP2; TIM8B
NCBI Protein Information:	mitochondrial import inner membrane translocase subunit Tim8 B; DDP-like protein; deafness dystonia protein 2
UniProt Protein Name:	Mitochondrial import inner membrane translocase subunit Tim8 B
UniProt Synonym Protein Names:	DDP-like protein; Deafness dystonia protein 2
UniProt Gene Name:	TIMM8B
UniProt Entry Name:	TIM8B_HUMAN

	Western blot analysis of extracts of various cell lines, using TIMM8B antibody (CAB15814) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using TIMM8B antibody (CAB15814) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using TIMM8B Polyclonal Antibody (CAB15814) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.