Cell Biology Antibodies 7

Anti-TIMM44 Antibody (CAB17446)

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SKU:
CAB17446
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TIMM44 Antibody
Antibody SKU:CAB17446
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 153-452 of human TIMM44 (NP_006342.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, K562, mouse liver, rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 153-452 of human TIMM44 (NP_006342.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AKTA KQSA ESVS KGGE KLGR TAAF RALS QGVE SVKK EIDD SVLG QTGP YRRP QRLR KRTE FAGD KFKE EKVF EPNE EALG VVLH KDSK WYQQ WKDF KENN VVFN RFFE MKMK YDES DNAF IRAS RALT DKVT DLLG GLFS KTEM SEVL TEIL RVDP AFDK DRFL KQCE NDII PNVL EAMI SGEL DILK DWCY EATY SQLA HPIQ QAKA LGLQ FHSR ILDI DNVD LAMG KMME QGPV LIIT FQAQ LVMV VRNP KGEV VEGD PDKV LRML YVWA LCRD QDEL NPYA AWRL LDIS ASST EQIL
Gene ID:10469
Uniprot:O43615
Cellular Location:
Calculated MW:51kDa
Observed MW:51kDa
Synonyms:TIM44, TIMM44
Background:This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma.
UniProt Code:O43615
NCBI GenInfo Identifier:33636719
NCBI Gene ID:10469
NCBI Accession:NP_006342.2
UniProt Secondary Accession:O43615,O35857, O35094,
UniProt Related Accession:O43615
Molecular Weight:51,356 Da
NCBI Full Name:mitochondrial import inner membrane translocase subunit TIM44
NCBI Synonym Full Names:translocase of inner mitochondrial membrane 44 homolog (yeast)
NCBI Official Symbol:TIMM44  
NCBI Official Synonym Symbols:TIM44  
NCBI Protein Information:mitochondrial import inner membrane translocase subunit TIM44; mitochondrial inner membrane translocase
UniProt Protein Name:Mitochondrial import inner membrane translocase subunit TIM44
UniProt Gene Name:TIMM44  
UniProt Entry Name:TIM44_HUMAN
Anti-TIMM44 Antibody (CAB17446)Western blot analysis of extracts of various cell lines, using TIMM44 antibody (CAB17446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-TIMM44 Antibody (CAB17446)Immunofluorescence analysis of L929 cells using TIMM44 antibody (CAB17446) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-TIMM44 Antibody (CAB17446)Immunofluorescence analysis of U-2 OS cells using TIMM44 antibody (CAB17446) at dilution of 1:100. Blue: DAPI for nuclear staining.
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