Cell Biology Antibodies 9

Anti-TIMELESS Antibody (CAB4261)

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SKU:
CAB4261
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TIMELESS Antibody
Antibody SKU:CAB4261
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 969-1208 of human TIMELESS (NP_003911.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:BxPC-3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 969-1208 of human TIMELESS (NP_003911.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EDLE EEEN LPEE DSEE EEEG GSEA EQVQ GSLV LSNE NLGQ SLHQ EGFS IPLL WLQN CLIR AADD REED GCSQ AVPL VPLT EENE EAME NEQF QQLL RKLG VRPP ASGQ ETFW RIPA KLSP TQLR RAAA SLSQ PEEE QKLQ PELQ PKVP GEQG SDEE HCKE HRAQ ALRA LLLA HKKK AGLA SPEE EDAV GKEP LKAA PKKR QLLD SDEE QEED EGRN RAPE LGAP GIQK KKRY QIED DEDD
Gene ID:8914
Uniprot:Q9UNS1
Cellular Location:Nucleus
Calculated MW:138kDa
Observed MW:160kDa
Synonyms:TIMELESS, TIM, TIM1, hTIM
Background:The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders.
UniProt Protein Function:TIMELESS: Required for normal progression of S-phase. Involved in the circadian rhythm autoregulatory loop. Negatively regulates CLOCK-NPAS2/BMAL1-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus. Promotes TIPIN nuclear localiZation. Involved in cell survival after DNA damage or replication stress. May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light. May also play an important role in epithelial cell morphogenesis and formation of branching tubules. Belongs to the timeless family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: microtubule cytoskeleton; nuclear chromatin; nucleolus; nucleus

Molecular Function:protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: circadian rhythm; mitosis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; regulation of cell proliferation; branching morphogenesis of a tube; response to abiotic stimulus; cell division; morphogenesis of an epithelium; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; detection of abiotic stimulus; lung development

NCBI Summary:The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. [provided by RefSeq, Feb 2014]
UniProt Code:Q9UNS1
NCBI GenInfo Identifier:296452931
NCBI Gene ID:8914
NCBI Accession:Q9UNS1.2
UniProt Secondary Accession:Q9UNS1,O94802, Q86VM1, Q8IWH3, B2ZAV0,
UniProt Related Accession:Q9UNS1
Molecular Weight:Calculated Molecular Weight: 138kDaObserved Molecular Weight: 160kDa
NCBI Full Name:Protein timeless homolog
NCBI Synonym Full Names:timeless circadian clock
NCBI Official Symbol:TIMELESS  
NCBI Official Synonym Symbols:TIM; TIM1; hTIM  
NCBI Protein Information:protein timeless homolog; Tof1 homolog; timeless circadian clock 1
UniProt Protein Name:Protein timeless homolog
Protein Family:TIMELESS-interacting protein
UniProt Gene Name:TIMELESS  
UniProt Entry Name:TIM_HUMAN
Anti-TIMELESS Antibody (CAB4261)Western blot analysis of extracts of BxPC-3 cells, using TIMELESS antibody (CAB4261) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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