Cardiovascular Antibodies
Anti-TIE1 Antibody (CAB3334)
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- SKU:
- CAB3334
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-TIE1 Antibody |
| Antibody SKU: | CAB3334 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human TIE1 (NP_005415.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | MCF-7, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human TIE1 (NP_005415.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IPQI LNMA SELE FNLE TMPR INCA AAGN PFPV RGSI ELRK PDGT VLLS TKAI VEPE KTTA EFEV PRLV LADS GFWE CRVS TSGG QDSR RFKV NVKV PPVP LAAP RLLT KQSR QLVV SPLV SFSG DGPI STVR LHYR PQDS TMDW STIV VDPS ENVT LMNL RPKT GYSV RVQL SRPG EGGE GAWG PPTL MTTD CPEP LLQP WLEG WHVE GTDR LRVS WSLP LVPG PLVG DGFL LRLW DGTR GQER RENV SSP |
| Gene ID: | 7075 |
| Uniprot: | P35590 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 34kDa/40kDa/125kDa |
| Observed MW: | 135/140kDa |
| Synonyms: | TIE1, JTK14, TIE |
| Background: | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family Chromosomal Location of Human Ortholog: 1p34-p33 Cellular Component: integral to plasma membrane Molecular Function:protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding Biological Process: negative regulation of angiogenesis; peptidyl-tyrosine phosphorylation; response to retinoic acid; in utero embryonic development; mesoderm development; angiogenesis; signal transduction; vasculogenesis; negative regulation of cell migration; plasma membrane fusion |
| NCBI Summary: | This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | P35590 |
| NCBI GenInfo Identifier: | 4885631 |
| NCBI Gene ID: | 7075 |
| NCBI Accession: | NP_005415.1 |
| UniProt Related Accession: | P35590 |
| Molecular Weight: | 125,090 Da |
| NCBI Full Name: | tyrosine-protein kinase receptor Tie-1 isoform 1 |
| NCBI Synonym Full Names: | tyrosine kinase with immunoglobulin-like and EGF-like domains 1 |
| NCBI Official Symbol: | TIE1 |
| NCBI Official Synonym Symbols: | TIE; JTK14 |
| NCBI Protein Information: | tyrosine-protein kinase receptor Tie-1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 |
| UniProt Protein Name: | Tyrosine-protein kinase receptor Tie-1 |
| UniProt Gene Name: | TIE1 |
| UniProt Entry Name: | TIE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIE1 antibody (CAB3334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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