Name: Anti-TICAM1 Antibody (CAB14162)
Brand: Immunology Antibodies 1
SKU: CAB14162
Availability: OutOfStock

Antibody Name:	Anti-TICAM1 Antibody
Antibody SKU:	CAB14162
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TICAM1 (NP_891549.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SKOV3

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 150-250 of human TICAM1 (NP_891549.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDPG SIRT LQSN LGCL PPSS ALPS GTRS LPRP IDGV SDWS QGCS LRST GSPA SLAS NLEI SQSP TMPF LSLH RSPH GPSK LCDD PQAS LVPE PVPG GCQE P
Gene ID:	148022
Uniprot:	Q8IUC6
Cellular Location:	Cytoplasmic vesicle, autophagosome
Calculated MW:	76kDa
Observed MW:	76kDa

Synonyms:

IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF, TRIF/TICAM1, TICAM1

Background:

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response.

UniProt Protein Function:	TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Apoptosis Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: endosome membrane; cytosol Molecular Function:protein binding; signal transducer activity; protein kinase binding Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; regulation of protein homodimerization activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; positive regulation of chemokine biosynthetic process; response to exogenous dsRNA; positive regulation of protein ubiquitination; macrophage activation during immune response; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; positive regulation of B cell proliferation; lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus Disease: Herpes Simplex Encephalitis, Susceptibility To, 4
NCBI Summary:	This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012]
UniProt Code:	Q8IUC6
NCBI GenInfo Identifier:	74727957
NCBI Gene ID:	148022
NCBI Accession:	Q8IUC6.1
UniProt Related Accession:	Q8IUC6
Molecular Weight:
NCBI Full Name:	TIR domain-containing adapter molecule 1
NCBI Synonym Full Names:	toll like receptor adaptor molecule 1
NCBI Official Symbol:	TICAM1
NCBI Official Synonym Symbols:	TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
NCBI Protein Information:	TIR domain-containing adapter molecule 1
UniProt Protein Name:	TIR domain-containing adapter molecule 1
UniProt Synonym Protein Names:	Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
Protein Family:	Trifolitoxin
UniProt Gene Name:	TICAM1
UniProt Entry Name:	TCAM1_HUMAN

Western blot analysis of extracts of SKOV3 cells, using TICAM1 antibody (CAB14162) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.

Antibodies

Anti-TRIF / TICAM1 Antibody (CAB1155)

Anti-TICAM1 Antibody (CAB13605)

TICAM1 Antibody (PACO07270)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)