Antibody Name:	Anti-TICAM1 Antibody
Antibody SKU:	CAB13605
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human TICAM1 (NP_891549.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, Mouse spleen, Mouse eye, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human TICAM1 (NP_891549.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QDKL LYLK HKLK TPRP GCQG QDLL HAMV LLKL GQET EARI SLEA LKAD AVAR LVAR QWAG VDST EDPE EPPD VSWA VARL YHLL AEEK LCPA SLRD VAYQ EAVR TLSS RDDH RLGE LQDE ARNR CGWD IAGD PGSI RTLQ SNLG CLPP SSAL PSGT RSLP RPID GVSD WSQG CSLR STGS P
Gene ID:	148022
Uniprot:	Q8IUC6
Cellular Location:	Cytoplasmic vesicle, autophagosome
Calculated MW:	76kDa
Observed MW:	70kDa

Synonyms:

IIAE6, MyD88-3, PRVTIRB, TICAM-1, TRIF, TRIF/TICAM1, TICAM1

Background:

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response.

UniProt Protein Function:	TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Apoptosis Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: endosome membrane; cytosol Molecular Function:protein binding; signal transducer activity; protein kinase binding Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; regulation of protein homodimerization activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; positive regulation of chemokine biosynthetic process; response to exogenous dsRNA; positive regulation of protein ubiquitination; macrophage activation during immune response; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; positive regulation of B cell proliferation; lipopolysaccharide-mediated signaling pathway; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus Disease: Herpes Simplex Encephalitis, Susceptibility To, 4
NCBI Summary:	This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012]
UniProt Code:	Q8IUC6
NCBI GenInfo Identifier:	74727957
NCBI Gene ID:	148022
NCBI Accession:	Q8IUC6.1
UniProt Related Accession:	Q8IUC6
Molecular Weight:
NCBI Full Name:	TIR domain-containing adapter molecule 1
NCBI Synonym Full Names:	toll like receptor adaptor molecule 1
NCBI Official Symbol:	TICAM1
NCBI Official Synonym Symbols:	TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
NCBI Protein Information:	TIR domain-containing adapter molecule 1
UniProt Protein Name:	TIR domain-containing adapter molecule 1
UniProt Synonym Protein Names:	Proline-rich, vinculin and TIR domain-containing protein B; Putative NF-kappa-B-activating protein 502H; Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta
Protein Family:	Trifolitoxin
UniProt Gene Name:	TICAM1
UniProt Entry Name:	TCAM1_HUMAN

	Western blot analysis of extracts of various cell lines, using TICAM1 Rabbit pAb (CAB13605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using TICAM1 antibody (CAB13605) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using TICAM1 antibody (CAB13605) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using TICAM1 antibody (CAB13605) at dilution of 1:100 (40x lens).