Epigenetics & Nuclear Signaling Antibodies 1

Anti-THOC3 Antibody (CAB13230)

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SKU:
CAB13230
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-THOC3 Antibody
Antibody SKU:CAB13230
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human THOC3 (NP_115737.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human THOC3 (NP_115737.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAVP AAAM GPSA LGQS GPGS MAPW CSVS SGPS RYVL GMQE LFRG HSKT REFL AHSA KVHS VAWS CDGR RLAS GSFD KTAS VFLL EKDR LVKE NNYR GHGD SVDQ LCWH PSNP DLFV TASG DKTI RIWD VRTT KCIA TVNT KGEN INIC WSPD GQTI AVGN KDDV VTFI DAKT HRSK AEEQ FKFE VNEI SWNN DNNM FFLT
Gene ID:84321
Uniprot:Q96J01
Cellular Location:Nucleus, Nucleus speckle
Calculated MW:36kDa/38kDa
Observed MW:39kDa
Synonyms:THOC3, THO3, hTREX45
Background:This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5.
UniProt Protein Function:THOC3: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex.The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4.
UniProt Protein Details:

Protein type:Spliceosome

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nuclear chromosome, telomeric region; nucleoplasm

Biological Process: intronless viral mRNA export from host nucleus; mRNA 3'-end processing; mRNA export from nucleus; RNA export from nucleus; termination of RNA polymerase II transcription

NCBI Summary:This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. [provided by RefSeq, May 2013]
UniProt Code:Q96J01
NCBI GenInfo Identifier:48474597
NCBI Gene ID:84321
NCBI Accession:Q96J01.1
UniProt Secondary Accession:Q96J01,Q6NZ53,
UniProt Related Accession:Q96J01
Molecular Weight:36,032 Da
NCBI Full Name:THO complex subunit 3
NCBI Synonym Full Names:THO complex 3
NCBI Official Symbol:THOC3  
NCBI Official Synonym Symbols:THO3; hTREX45  
NCBI Protein Information:THO complex subunit 3
UniProt Protein Name:THO complex subunit 3
UniProt Synonym Protein Names:TEX1 homolog; hTREX45
UniProt Gene Name:THOC3  
UniProt Entry Name:THOC3_HUMAN
Anti-THOC3 Antibody (CAB13230)Western blot analysis of extracts of various cell lines, using THOC3 antibody (CAB13230) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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