Name: Anti-THEM4 Antibody (CAB15940)
Brand: Cell Death Antibodies 1
SKU: CAB15940
Availability: OutOfStock

Antibody Name:	Anti-THEM4 Antibody
Antibody SKU:	CAB15940
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 37-150 of human THEM4 (NP_444283.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-87MG, HeLa, Mouse brain, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 37-150 of human THEM4 (NP_444283.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSEE VILK DCSV PNPS WNKD LRLL FDQF MKKC EDGS WKRL PSYK RTPT EWIQ DFKT HFLD PKLM KEEQ MSQA QLFT RSFD DGLG FEYV MFYN DIEK RMVC LFQG GPYL EGPP GF
Gene ID:	117145
Uniprot:	Q5T1C6
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein, ruffle membrane
Calculated MW:	27kDa
Observed MW:	25kDa/27kDa

Synonyms:

THEM4, CTMP

Background:

Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas.

UniProt Protein Function:	CTMP: According to PubMed:11598301, inhibits AKT1 phosphorylation and activity. According to PubMed:17615157, enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane. Interacts with AKT1. Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation.
UniProt Protein Details:	Protein type:EC 3.1.2.2 Chromosomal Location of Human Ortholog: 1q21 Cellular Component: mitochondrion; mitochondrial inner membrane; plasma membrane; mitochondrial intermembrane space; cytosol Molecular Function:palmitoyl-CoA hydrolase activity Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; protein kinase B signaling cascade; insulin receptor signaling pathway; innate immune response; fatty acid metabolic process
NCBI Summary:	Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. [provided by RefSeq, Jul 2008]
UniProt Code:	Q5T1C6
NCBI GenInfo Identifier:	76159293
NCBI Gene ID:	117145
NCBI Accession:	NP_444283
UniProt Secondary Accession:	Q5T1C6,Q3UUI3,
UniProt Related Accession:	Q5T1C6
Molecular Weight:	27,130 Da
NCBI Full Name:	acyl-coenzyme A thioesterase THEM4
NCBI Synonym Full Names:	thioesterase superfamily member 4
NCBI Official Symbol:	THEM4
NCBI Official Synonym Symbols:	CTMP
NCBI Protein Information:	acyl-coenzyme A thioesterase THEM4; acyl-CoA thioesterase THEM4; C-terminal modulator protein; carboxyl-terminal modulator protein
UniProt Protein Name:	Acyl-coenzyme A thioesterase THEM4
UniProt Synonym Protein Names:	Carboxyl-terminal modulator protein; Thioesterase superfamily member 4
UniProt Gene Name:	THEM4
UniProt Entry Name:	THEM4_HUMAN

Western blot analysis of extracts of various cell lines, using THEM4 antibody (CAB15940) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

THEM4 Antibody (PACO12738)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)