Name: Anti-THAP1 Antibody (CAB7472)
Brand: Cell Cycle Antibodies 2
SKU: CAB7472
Availability: OutOfStock

Antibody Name:	Anti-THAP1 Antibody
Antibody SKU:	CAB7472
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human THAP1 (NP_060575.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse liver, Mouse thymus, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human THAP1 (NP_060575.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVQS CSAY GCKN RYDK DKPV SFHK FPLT RPSL CKEW EAAV RRKN FKPT KYSS ICSE HFTP DCFK RECN NKLL KENA VPTI FLCT EPHD KKED LLEP QEQL PPPP LPPP VSQV DAAI GLLM PPLQ TPVN LSVF CDHN YTVE DTMH QRKR IHQL EQQV EKLR KKLK TAQQ RCRR QERQ LEKL KEVV HFQK EKDD VSER GYVI LPND YFEI VEVP A
Gene ID:	55145
Uniprot:	Q9NVV9
Cellular Location:	Nucleus, PML body, nucleoplasm
Calculated MW:	6kDa/24kDa
Observed MW:	25kDa

Synonyms:	THAP1, DYT6
Background:	The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	THAP1: DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Defects in THAP1 are the cause of dystonia type 6 (DYT6). DYT6 is a primary torsion dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Dystonia type 6 is characterized by onset in early adulthood, cranial or cervical involvement in about half of the cases, and frequent progression to involve multiple body regions. Belongs to the THAP1 family.
UniProt Protein Details:	Protein type:DNA-binding; Cell cycle regulation; Transcription factor Chromosomal Location of Human Ortholog: 8p11.21 Cellular Component: PML body; nucleus Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; endothelial cell proliferation; regulation of mitotic cell cycle; cell cycle Disease: Dystonia 6, Torsion
NCBI Summary:	The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NVV9
NCBI GenInfo Identifier:	29839656
NCBI Gene ID:	55145
NCBI Accession:	Q9NVV9.1
UniProt Secondary Accession:	Q9NVV9,Q53FQ1, Q6IA99, A6NCB6, D3DSY5, H9KV49,
UniProt Related Accession:	Q9NVV9
Molecular Weight:	213
NCBI Full Name:	THAP domain-containing protein 1
NCBI Synonym Full Names:	THAP domain containing, apoptosis associated protein 1
NCBI Official Symbol:	THAP1
NCBI Official Synonym Symbols:	DYT6
NCBI Protein Information:	THAP domain-containing protein 1; 4833431A01Rik; THAP domain protein 1; nuclear proapoptotic factor
UniProt Protein Name:	THAP domain-containing protein 1
Protein Family:	THAP domain-containing protein
UniProt Gene Name:	THAP1
UniProt Entry Name:	THAP1_HUMAN

	Western blot analysis of extracts of various cell lines, using THAP1 antibody (CAB7472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat heart using THAP1 antibody (CAB7472) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using THAP1 antibody (CAB7472) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using THAP1 antibody (CAB7472).