Name: Anti-TGM5 Antibody (CAB7039)
Brand: Cell Biology Antibodies 10
SKU: CAB7039
Availability: OutOfStock

Antibody Name:	Anti-TGM5 Antibody
Antibody SKU:	CAB7039
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse heart, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 461-720 of human TGM5 (NP_963925.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LQKL KARS FHGS QRGA ELQP SRPT SLSQ DSPR SLHT PSLR PSDV VQVS LKFK LLDP PNMG QDIC FVLL ALNM SSQF KDLK VNLS AQSL LHDG SPLS PFWQ DTAF ITLS PKEA KTYP CKIS YSQY SQYL STDK LIRI SALG EEKS SPEK ILVN KIIT LSYP SITI NVLG AAVV NQPL SIQV IFSN PLSE QVED CVLT VEGS GLFK KQQK VFLG VLKP QHQA SIIL ETVP FKSG QRQI QANM RSNK FKDI KGYR NVYV DFAL
Gene ID:	9333
Uniprot:	O43548
Cellular Location:	Cytoplasm
Calculated MW:	71kDa/80kDa
Observed MW:	81kDa

Synonyms:	TGM5, PSS2, TGASE5, TGASEX, TGM6, TGMX, TGX
Background:	This gene encodes a member of the transglutaminase family. The encoded protein catalyzes formation of protein cross-links between glutamine and lysine residues, often resulting in stabilization of protein assemblies. This reaction is calcium dependent. Mutations in this gene have been associated with acral peeling skin syndrome.

UniProt Protein Function:	TGM5: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Contributes to the formation of the cornified cell envelope of keratinocytes. Defects in TGM5 are a cause of peeling skin syndrome type A (APSS). A non-inflammatory form of peeling skin syndrome, a genodermatosis characterized by the continuous shedding of the outer layers of the epidermis. In APSS patients, skin peeling is strictly limited to the dorsa of the hands and feet, and it is accompanied by accompanied by painless erythema and spontaneous non-scarring healing. Ultrastructural and histological analysis shows a level of blistering high in the epidermis at the stratum granulosum-stratum corneum junction. Belongs to the transglutaminase superfamily. Transglutaminase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; EC 2.3.2.13 Chromosomal Location of Human Ortholog: 15q15.2 Cellular Component: cytoplasm Molecular Function:protein-glutamine gamma-glutamyltransferase activity; metal ion binding Biological Process: epidermis development; protein modification process; peptide cross-linking Disease: Peeling Skin Syndrome 2
NCBI Summary:	This gene encodes a member of the transglutaminase family. The encoded protein catalyzes formation of protein cross-links between glutamine and lysine residues, often resulting in stabilization of protein assemblies. This reaction is calcium dependent. Mutations in this gene have been associated with acral peeling skin syndrome. [provided by RefSeq, Oct 2009]
UniProt Code:	O43548
NCBI GenInfo Identifier:	20532415
NCBI Gene ID:	9333
NCBI Accession:	O43548.4
UniProt Secondary Accession:	O43548,O43549, Q0VF40, Q9UEZ4,
UniProt Related Accession:	O43548
Molecular Weight:	720
NCBI Full Name:	Protein-glutamine gamma-glutamyltransferase 5
NCBI Synonym Full Names:	transglutaminase 5
NCBI Official Symbol:	TGM5
NCBI Official Synonym Symbols:	TGX; PSS2; TGM6; TGMX; TGASE5; TGASEX
NCBI Protein Information:	protein-glutamine gamma-glutamyltransferase 5; TG(X); TGase X; TGase-5; transglutaminase V; transglutaminase X; transglutaminase-5
UniProt Protein Name:	Protein-glutamine gamma-glutamyltransferase 5
UniProt Synonym Protein Names:	Transglutaminase X; TG(X); TGX; TGase X; Transglutaminase-5; TGase-5
Protein Family:	Protein-glutamine gamma-glutamyltransferase
UniProt Gene Name:	TGM5
UniProt Entry Name:	TGM5_HUMAN

	Western blot analysis of extracts of various cell lines, using TGM5 antibody (CAB7039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat kidney using TGM5 antibody (CAB7039) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using TGM5 antibody (CAB7039) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using TGM5 antibody (CAB7039) at dilution of 1:200 (40x lens).