Antibody Name:	Anti-TGM3 Antibody
Antibody SKU:	CAB5856
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	BT-474, A-549, U-87MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TGM3 (NP_003236.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL GVQS INWQ TAFN RQAH HTDK FSSQ ELIL RRGQ NFQV LMIM NKGL GSNE RLEF IVST GPYP SESA MTKA VFPL SNGS SGGW SAVL QASN GNTL TISI SSPA SAPI GRYT MALQ IFSQ GGIS SVKL GTFI LLFN PWLN VDSV FMGN HAER EEYV QEDA GIIF VGST NRIG MIGW NFGQ
Gene ID:	7053
Uniprot:	Q08188
Cellular Location:
Calculated MW:	76kDa
Observed MW:	72kDa

Synonyms:

TGM3, TGE, UHS2

Background:

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle.

UniProt Protein Function:	TGM3: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross- linking structural proteins to hardening the inner root sheath. Belongs to the transglutaminase superfamily. Transglutaminase family.
UniProt Protein Details:	Protein type:EC 2.3.2.13; Membrane protein, peripheral; Transferase Chromosomal Location of Human Ortholog: 20q11.2 Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm Molecular Function:protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups; calcium ion binding; catalytic activity Biological Process: keratinocyte differentiation; cell envelope organization and biogenesis; hair follicle morphogenesis; keratinization; protein modification process; peptide cross-linking; protein tetramerization
NCBI Summary:	Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]
UniProt Code:	Q08188
NCBI GenInfo Identifier:	257051080
NCBI Gene ID:	7053
NCBI Accession:	Q08188.4
UniProt Secondary Accession:	Q08188,O95933, Q32ML9, Q32MM0, A8K5N6, B2RCR6, D3DVX1
UniProt Related Accession:	Q08188
Molecular Weight:
NCBI Full Name:	Protein-glutamine gamma-glutamyltransferase E
NCBI Synonym Full Names:	transglutaminase 3
NCBI Official Symbol:	TGM3
NCBI Official Synonym Symbols:	TGE
NCBI Protein Information:	protein-glutamine gamma-glutamyltransferase E; TG(E); TGase E; TGase-3; transglutaminase E; transglutaminase-3; E polypeptide, protein-glutamine-gamma-glutamyltransferase
UniProt Protein Name:	Protein-glutamine gamma-glutamyltransferase E
UniProt Synonym Protein Names:	Transglutaminase E; TG(E); TGE; TGase E; Transglutaminase-3; TGase-3
Protein Family:	Protein-glutamine gamma-glutamyltransferase
UniProt Gene Name:	TGM3
UniProt Entry Name:	TGM3_HUMAN

	Western blot analysis of extracts of various cell lines, using TGM3 antibody (CAB5856) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 50s.
	Immunofluorescence analysis of U2OS cells using TGM3 antibody (CAB5856). Blue: DAPI for nuclear staining.