Cell Biology Antibodies 3

Anti-TGM1 Antibody (CAB13359)

(No reviews yet) Write a Review
SKU:
CAB13359
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-TGM1 Antibody
Antibody SKU:CAB13359
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, Mouse kidney, Rat kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AGDG TIRE GMLV VNGV DLLS SRSD QNRR EHHT DEYE YDEL IVRR GQPF HMLL LLSR TYES SDRI TLEL LIGN NPEV GKGT HVII PVGK GGSG GWKA QVVK ASGQ NLNL RVHT SPNA IIGK FQFT VRTQ SDAG EFQL PFDP RNEI YILF NPWC PEDI VYVD HEDW RQEY VLNE SGRI YYGT E
Gene ID:7051
Uniprot:P22735
Cellular Location:Lipid-anchor, Membrane
Calculated MW:40kDa/89kDa
Observed MW:90kDa
Synonyms:TGM1, ARCI1, ICR2, KTG, LI, LI1, TGASE, TGK
Background:The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE).
UniProt Protein Function:TGM1: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Responsible for cross-linking epidermal proteins during formation of the stratum corneum.
UniProt Protein Details:

Protein type:Transferase; EC 2.3.2.13

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: cell-cell adherens junction; cornified envelope; intrinsic to membrane

Molecular Function:protein binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: cell envelope organization and biogenesis; keratinocyte differentiation; organ morphogenesis; keratinization; protein modification process; peptide cross-linking

Disease: Ichthyosis, Congenital, Autosomal Recessive 1

NCBI Summary:The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008]
UniProt Code:P22735
NCBI GenInfo Identifier:57015359
NCBI Gene ID:7051
NCBI Accession:P22735.4
UniProt Related Accession:P22735
Molecular Weight:Observed: 90 kDaPredicted: 90 kDa
NCBI Full Name:Protein-glutamine gamma-glutamyltransferase K
NCBI Synonym Full Names:transglutaminase 1
NCBI Official Symbol:TGM1  
NCBI Official Synonym Symbols:LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE  
NCBI Protein Information:protein-glutamine gamma-glutamyltransferase K
UniProt Protein Name:Protein-glutamine gamma-glutamyltransferase K
UniProt Synonym Protein Names:Epidermal TGase; Transglutaminase K; TG(K); TGK; TGase K; Transglutaminase-1; TGase-1
Protein Family:Protein-glutamine gamma-glutamyltransferase
UniProt Gene Name:TGM1  
UniProt Entry Name:TGM1_HUMAN
Anti-TGM1 Antibody (CAB13359)Western blot analysis of extracts of various cell lines, using TGM1 antibody (CAB13359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-TGM1 Antibody (CAB13359)Immunofluorescence analysis of HeLa cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-TGM1 Antibody (CAB13359)Immunofluorescence analysis of rat skin cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-TGM1 Antibody (CAB13359)Immunofluorescence analysis of mouse skin cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products