Cell Biology Antibodies 14

Anti-TGIF1 Antibody (CAB16984)

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SKU:
CAB16984
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-TGIF1 Antibody (CAB16984)
Antibody SKU:CAB16984
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-340 of human TGIF1 (NP_733796.2).
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, K-562, U-251MG, Mouse liver, Mouse testis, Rat uterus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 250-340 of human TGIF1 (NP_733796.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SSVE SVMG IKNF MPAL EETP FHSC TAGP NPTL GRPL SPKP SSPG SVLA RPSV ICHT TVTA LKDV PFSL CQSV GVGQ NTDI QQIA AKNF TDT
Cellular Location:
Calculated MW:
Observed MW:35KDa
Synonyms:TGIF1, HPE4, TGIF
Background:The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described.
Western blot analysis of extracts of various cell lines, using TGIF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using TGIF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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