Cell Biology Antibodies 1
Anti-TGFA Antibody (CAB0337)
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- SKU:
- CAB0337
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TGFA Antibody |
| Antibody SKU: | CAB0337 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-98 of human TGFA (NP_001093161.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, 293T, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-98 of human TGFA (NP_001093161.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ENST SPLS DPPV AAAV VSHF NDCP DSHT QFCF HGTC RFLV QEDK PACV CHSG YVGA RCEH ADLL AVVA ASQK KQA |
| Gene ID: | 7039 |
| Uniprot: | P01135 |
| Cellular Location: | Cell membrane, Secreted, Single-pass type I membrane protein, extracellular space |
| Calculated MW: | 16kDa/17kDa |
| Observed MW: | 21kDa |
| Synonyms: | TGFA, TFGA |
| Background: | This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Ligand, receptor tyrosine kinase; Membrane protein, integral; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2p13 Cellular Component: extracellular space; cell surface; basolateral plasma membrane; perinuclear region of cytoplasm; integral to plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus Molecular Function:protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding Biological Process: response to drug; epidermal growth factor receptor signaling pathway; cell proliferation; positive regulation of epidermal growth factor receptor activity; wound healing; positive regulation of mitosis; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P01135 |
| NCBI GenInfo Identifier: | 135689 |
| NCBI Gene ID: | 7039 |
| NCBI Accession: | P01135.1 |
| UniProt Secondary Accession: | P01135,Q15577, Q53SK7, Q9BS56, Q9UEI3, Q9UKM1, Q9UKM2 Q9UKM3, A8K286, |
| UniProt Related Accession: | P01135 |
| Molecular Weight: | 17,329 Da |
| NCBI Full Name: | Protransforming growth factor alpha |
| NCBI Synonym Full Names: | transforming growth factor, alpha |
| NCBI Official Symbol: | TGFA |
| NCBI Official Synonym Symbols: | TFGA |
| NCBI Protein Information: | protransforming growth factor alpha; TGF-alpha |
| UniProt Protein Name: | Protransforming growth factor alpha |
| UniProt Synonym Protein Names: | EGF-like TGF; ETGF; TGF type 1 |
| Protein Family: | Transforming growth factor |
| UniProt Gene Name: | TGFA |
| UniProt Entry Name: | TGFA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TGFA antibody (CAB0337) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |
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